Team:Chiba/Notebook 3

From 2010.igem.org

(Difference between revisions)
Line 215: Line 215:
dH<sub>2</sub>O 5 µL
dH<sub>2</sub>O 5 µL
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--------------------
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 +
gel extraction
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<br>
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<html><h3>2010-10-11</h3></html>
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Ligation
 +
 +
R0061 (70 ng)  1 µL     
 +
E0240 (100 ng) 2 µL
 +
ATP            1 µL
 +
10×Buffer    1 µL
 +
T4 DNA Ligase  1 µL
 +
dH<sub>2</sub>O 4 µL
 +
 +
transformation
 +
 +
<html><h3>2010-10-12</h3></html>
 +
 +
Ligation
 +
 +
R0061(100 ng)  1 µL
 +
E0240 (100 ng) 3 µL
 +
ATP (10 mM)    1 µL
 +
T4 DNA Ligase  1 µL
 +
dH<sub>2</sub>O 4 µL
 +
 +
<html><h3>2010-10-13</h3></html>
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 +
 +
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 +
　

Revision as of 06:12, 26 October 2010




 


Lux inverter

2010-10-8


transformation

BBa_R0061 BBa_E0240


2010-10-9

Mini-prep BBa_R0061 BBa_E0240

digestion

BBa_R0061 20 µL 10×Buffer 3 µL SpeI　　　　　 1 µL PstI　　　　　 1 µL dH2O5 µL

BBa_E0240 20 µL 10×Buffer 3 µL XbaI 1 µL PstI　　　　　 1 µL dH2O 5 µL

gel extraction


2010-10-11

Ligation

R0061 (70 ng) 1 µL E0240 (100 ng) 2 µL ATP 1 µL 10×Buffer 1 µL T4 DNA Ligase 1 µL dH2O 4 µL

transformation

2010-10-12

Ligation

R0061(100 ng) 1 µL E0240 (100 ng) 3 µL ATP (10 mM) 1 µL T4 DNA Ligase 1 µL dH2O 4 µL

2010-10-13



　

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