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Team:Alberta/Kit
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'''The Innovation'''<p> | '''The Innovation'''<p> | ||
The limitations imposed by the high school requirements forced our team to design a new standard for the assembly and production of DNA parts, [[Team:Alberta/BioByte 2 |BioBytes 2.0]]. The BioByte 2.0 standard solved most of the problems.</p><p>BioBytes 2.0 is: | The limitations imposed by the high school requirements forced our team to design a new standard for the assembly and production of DNA parts, [[Team:Alberta/BioByte 2 |BioBytes 2.0]]. The BioByte 2.0 standard solved most of the problems.</p><p>BioBytes 2.0 is: | ||
| - | *Fast | + | *Fast: Parts can be added to a growing construct in 7 minutes. |
*Efficient-It has been modelled to be 96% efficient. We have also used BioBytes 2.0 to create plasmids that have been transformed successfully into ''E. coli''. | *Efficient-It has been modelled to be 96% efficient. We have also used BioBytes 2.0 to create plasmids that have been transformed successfully into ''E. coli''. | ||
*Equipment-It does not require centrifugation or any other expensive equipment inherently. | *Equipment-It does not require centrifugation or any other expensive equipment inherently. | ||
Revision as of 03:09, 26 October 2010
GENOMIKON
The Idea
GENOMIKON evolved from a team objective to bring synthetic biology to high school students. This proved to be a tall task as a high school environment has many limitations that don't apply to university research labs.
To work GENOMIKON must be:
- Require very little expensive equipment (autoclaves, centrifuges, -70C freezers)
- Fast
- Efficicent
- Easily shipped
- Inexpensive
The limitations imposed by the high school requirements forced our team to design a new standard for the assembly and production of DNA parts, BioBytes 2.0. The BioByte 2.0 standard solved most of the problems.
BioBytes 2.0 is:
- Fast: Parts can be added to a growing construct in 7 minutes.
- Efficient-It has been modelled to be 96% efficient. We have also used BioBytes 2.0 to create plasmids that have been transformed successfully into E. coli.
- Equipment-It does not require centrifugation or any other expensive equipment inherently.
The other limitations were solved by using innovative protocols and products:
- Equipment-Expensive micropipetters are replaced with precision droppers, which can accurately dispense small volumes of solutions.
- Easily Shipped-We will utilize lyophilized competent cells so that cold temperatures are not neccesary.
- Inexpensive-Based on our distribution analysis the kit will be inexpensive to produce and can be sold at costs that are lower than currently used high school biology labs.
[picture of kit buddi]
SafetyGENOMIKON was designed with safety in mind and as such it has many safety features built in.
- A common lab strain of E. coli DH5α is used. This strain has been used safely in labs around the world and was designed to be safe.
- Proper safety equipment is not required but will be recommended to further decrease the risks of contamination.
- The kit will teach safety education to the students.
When we tested our kit with high school students from the Edmonton area it had a warm reception. They had great things to say about the educational aspect and the scientfic aspect of the kit. They did give us some useful feedback. An in-depth look at this component can be seen on real world trials.
