Team:Chiba/Notebook 3

From 2010.igem.org

(Difference between revisions)
Line 183: Line 183:
'''Lux inverter'''
'''Lux inverter'''
-
BBa_R0061&BBa_E0240
+
2010 10 6
 +
 
 +
transformation
 +
--------------------
 +
BBa_R0061
 +
BBa_E0240
 +
--------------------
 +
 
 +
2010 10 7
 +
digestion
 +
--------------------
 +
BBa_R0061    20 µL
 +
10×Buffer    3 µL
 +
SpeI　　　　　 1 µL
 +
PstI　　　　　 1 µL
 +
dH<sub>2</sub>O5 µL
 +
--------------------
 +
 
 +
--------------------
 +
BBa_E0240      20 µL
 +
10×Buffer      3 µL
 +
XbaI            1 µL
 +
PstI　　　　　  1 µL
 +
dH<sub>2</sub>O 5 µL
 +
--------------------

Revision as of 02:52, 26 October 2010




 

Lux inverter

2010 10 6

transformation

BBa_R0061 BBa_E0240

2010 10 7 digestion

BBa_R0061 20 µL 10×Buffer 3 µL SpeI　　　　　 1 µL PstI　　　　　 1 µL dH2O5 µL

BBa_E0240 20 µL 10×Buffer 3 µL XbaI 1 µL PstI　　　　　 1 µL dH2O 5 µL

Retrieved from "http://2010.igem.org/Team:Chiba/Notebook_3"