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Team:Newcastle/3 September 2010
From 2010.igem.org
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* '''Lane 1''': 1kb DNA ladder | * '''Lane 1''': 1kb DNA ladder | ||
| - | * '''Lane 2''': | + | * '''Lane 2''': Tube 1 |
| - | * '''Lane 3''': | + | * '''Lane 3''': Tube 2 |
| - | * '''Lane 4''': | + | * '''Lane 4''': Tube 3 |
| - | * '''Lane 5''': | + | * '''Lane 5''': Tube 4 |
| - | * '''Lane 6''': | + | * '''Lane 6''': Tube 5 |
| - | * '''Lane 7''': | + | * '''Lane 7''': Tube 6 |
| - | * '''Lane 8''': | + | * '''Lane 8''': Tube 7 |
| - | * '''Lane 9''': | + | * '''Lane 9''': Tube 8 |
| - | * '''Lane 10''': | + | * '''Lane 10''': Tube 9 |
| - | * '''Lane 11''': | + | * '''Lane 11''': Tube 10 |
| - | * '''Lane 12''': | + | * '''Lane 12''': Tube 11 |
| - | * '''Lane 13''': | + | * '''Lane 13''': Tube 12 |
* '''Lane 14''': 1kb DNA ladder | * '''Lane 14''': 1kb DNA ladder | ||
Revision as of 00:48, 26 October 2010
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Contents |
yneA
Aims
The aim of the experiment is to test for the correct integration of yneA in pGFPrrnb plasmid.
Materials and protocols
- Please refer to: Plasmid extraction.
- Please refer to:Restriction digest. We used Nhe1 and Spe1 to remove the yneA from pGFP-rrnB.
- Please refer to: Gel electrophoresis for running all the digested plasmid fragments.
Results
Figure 1:
- Lane 1: 1kb DNA ladder
- Lane 2: Tube 1
- Lane 3: Tube 2
- Lane 4: Tube 3
- Lane 5: Tube 4
- Lane 6: Tube 5
- Lane 7: Tube 6
- Lane 8: Tube 7
- Lane 9: Tube 8
- Lane 10: Tube 9
- Lane 11: Tube 10
- Lane 12: Tube 11
- Lane 13: Tube 12
- Lane 14: 1kb DNA ladder
Conclusion
The results show that the digest works, there is a band at approximately 541bp corresponding to yneA and a band at approximately 8.4kbp corresponding to pGFPrrnb in lanes 2,3,4,6,7,8,9, 11 and 12.
   
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