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Team:Newcastle/3 September 2010
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[[Image:03.09.10.png#file|400px]] | [[Image:03.09.10.png#file|400px]] | ||
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| + | '''Figure 1''': | ||
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| + | * '''Lane 1''': 1kb DNA ladder | ||
| + | * '''Lane 2''': Plamid pMutin4 containing ''lacI'' | ||
| + | * '''Lane 3''': Plamid pMutin4 containing Pspac_oid promoter | ||
| + | * '''Lane 4''': 100bp DNA ladder | ||
| + | * '''Lane 5''': | ||
| + | * '''Lane 6''': | ||
| + | * '''Lane 7''': | ||
| + | * '''Lane 8''': | ||
| + | * '''Lane 9''': | ||
| + | * '''Lane 10''': | ||
| + | * '''Lane 11''': | ||
| + | * '''Lane 12''': | ||
| + | * '''Lane 13''': | ||
| + | * '''Lane 14''': | ||
==Conclusion== | ==Conclusion== | ||
Revision as of 00:45, 26 October 2010
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Contents |
yneA
Aims
The aim of the experiment is to test for the correct integration of yneA in pGFPrrnb plasmid.
Materials and protocols
- Please refer to: Plasmid extraction.
- Please refer to:Restriction digest. We used Nhe1 and Spe1 to remove the yneA from pGFP-rrnB.
- Please refer to: Gel electrophoresis for running all the digested plasmid fragments.
Results
Figure 1:
- Lane 1: 1kb DNA ladder
- Lane 2: Plamid pMutin4 containing lacI
- Lane 3: Plamid pMutin4 containing Pspac_oid promoter
- Lane 4: 100bp DNA ladder
- Lane 5:
- Lane 6:
- Lane 7:
- Lane 8:
- Lane 9:
- Lane 10:
- Lane 11:
- Lane 12:
- Lane 13:
- Lane 14:
Conclusion
The results show that the digest works, there is a band at approximately 541bp corresponding to yneA and a band at approximately 8.4kbp corresponding to pGFPrrnb in lanes 2,3,4,6,7,8,9, 11 and 12.
   
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