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Team:Newcastle/Meetings/26 March 2010
From 2010.igem.org
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#*Recombination site... Find!!! | #*Recombination site... Find!!! | ||
#*amyE site amylase with insert can’t break down amylase | #*amyE site amylase with insert can’t break down amylase | ||
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#'''Nutrients''' (Harsh and Rachel) | #'''Nutrients''' (Harsh and Rachel) | ||
#*Bacteria algae growth living in concrete | #*Bacteria algae growth living in concrete | ||
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#*Biofilms exclude other not algae | #*Biofilms exclude other not algae | ||
#*Antifungals, antialgaes, antibiotic | #*Antifungals, antialgaes, antibiotic | ||
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#'''Sporulation, germination and secretion control''' (Alan and Da) | #'''Sporulation, germination and secretion control''' (Alan and Da) | ||
#*SpoA – last years team sporulation control, maybe turn off completely | #*SpoA – last years team sporulation control, maybe turn off completely | ||
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#*Inducible promoter | #*Inducible promoter | ||
#*Glue out of bug | #*Glue out of bug | ||
| - | + | #'''Quorum sensing''' (Younus and Phil) | |
| - | '''Quorum sensing''' (Younus and Phil) | + | #*CompX and cff |
| - | *CompX and cff | + | #*Two-component system comP |
| - | *Two-component system comP | + | #'''Kill switch''' (Richard and Jannetta) |
| - | + | #*Why ? Don’t want?- not die after job fill new cracks | |
| - | '''Kill switch''' (Richard and Jannetta) | + | #*Nuclease |
| - | *Why ? Don’t want?- not die after job fill new cracks | + | #*Phil masEF |
| - | *Nuclease | + | #*Skf SbB |
| - | *Phil masEF | + | #*If linear DNA works |
| - | *Skf SbB | + | #'''Glue, CaCo3 and spider silk''' (Zoltan and Jannetta) |
| - | *If linear DNA works | + | #*Bacteria that produce CaCo3 from urea |
| - | + | #*How secrete spider silk long complex proteins – how can we make it happen in bacillus | |
| - | + | #*Secretion systems | |
| - | '''Glue, CaCo3 and spider silk''' (Zoltan and Jannetta) | + | #'''Other points''' |
| - | *Bacteria that produce CaCo3 from urea | + | #*Linear DNA – Richard |
| - | *How secrete spider silk long complex proteins – how can we make it happen in bacillus | + | #*Justifications of Linear |
| - | *Secretion systems | + | #*Build it up the use. |
| - | + | #*Mutations- How stop general questions | |
| - | '''Other points''' | + | #*Not specifically a problem – keep thinking about it though |
| - | *Linear DNA – Richard | + | |
| - | Justifications of Linear | + | |
| - | Build it up the use. | + | |
| - | Mutations- How stop general questions | + | |
| - | Not specifically a problem – keep thinking about it though | + | |
====Action points==== | ====Action points==== | ||
Revision as of 00:44, 26 October 2010
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Contents |
Formal meeting - 26th March 2010
- Room: CBCB; Start: 11 am
- Chair: Jannetta, Minutes: Rachel, Computer: Harsh
- Present: Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh
- Apologies: Phil (Holiday), Jem and Matt
- Approval of agenda and minutes - Approved
Research Feedback
- Properties of concrete and surface tension (Zoltan and Steven)
- High compressive strength low tensile strength
- Steal added good for temp change
- CaCO3 – similar to concrete; expand same as concrete... might crack like concrete
- Spider silk like steal- reduce possibility of cracking
- Glue- no many papers- expansion may not be a problem?
- Silk alkali resistant
- Capillary properties of concrete change over time- in project we consider small rapid
- Cement water ratio and surface area and environment
- Where to look for the cracks? Crack pattern
- Microinjection (Zoltan and Steven)
- Simple issue
- Not correct term
- Swimming Bacillus
- Oxygen gradient big selling points
- Genetics Homologous recombination plasmid chromosome (Harsh and Rachel)
- Test in plasmids- Find good plasmid
- Integrate into genome
- Recombination site... Find!!!
- amyE site amylase with insert can’t break down amylase
- Nutrients (Harsh and Rachel)
- Bacteria algae growth living in concrete
- Only activate feed bacillus
- Two hybrid
- Biofilms exclude other not algae
- Antifungals, antialgaes, antibiotic
- Sporulation, germination and secretion control (Alan and Da)
- SpoA – last years team sporulation control, maybe turn off completely
- Nutrient availability
- KinA
- Inducible promoter
- Glue out of bug
- Quorum sensing (Younus and Phil)
- CompX and cff
- Two-component system comP
- Kill switch (Richard and Jannetta)
- Why ? Don’t want?- not die after job fill new cracks
- Nuclease
- Phil masEF
- Skf SbB
- If linear DNA works
- Glue, CaCo3 and spider silk (Zoltan and Jannetta)
- Bacteria that produce CaCo3 from urea
- How secrete spider silk long complex proteins – how can we make it happen in bacillus
- Secretion systems
- Other points
- Linear DNA – Richard
- Justifications of Linear
- Build it up the use.
- Mutations- How stop general questions
- Not specifically a problem – keep thinking about it though
Action points
- All - Read Voygt paper
- All - Next week working off the proper wiki
- Janetta contact details doc in drop box
- Janetta and Richard – Flow chart!!!
- Reminder to neil and jen not welcome trust funding by 2nd of April?
- Janetta- T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper , quotes dependent on design
- Jannetta- send us links for graphic design software- please
- Transfer the wiki
- Individual iGEM applications - Phil hasn’t applied; Richard not accepted; Janetta text Phil
- Jen/Neil - Pay for team!!
- Visas/Passport update - Harsh will apply May/June; Richard needs to get a visa waiver
Wiki transfer
- Clarify contents of identified pages
- Ethics page
- Research page
- Safety page
- Identify other pages required
- Ideas for layout/theme
- Zoltan Tidy it up-Urgent
- Borrow last years / Look at anything thats nice
- It evolves!!! We can change it!!! Now!!!!!!!
Travel plans
- Flights and Accomodation
- Need to sort out who is going
Funding
- Harsh has made draft letter
- Main chemical company – free stuff?
Advisors/additional team members - civil engineer/graphics designer
- Still looking – Neil,Jen
Tutorials
- Sequentia and Clone manager - basics
- Modelling part two
What next?
- Flow chart of what we’re going to do Big picture!!!
- If its coloured life is easier
- Cambridge brick - Colour
- Web services for Biosynthetics, Flow chart
- Taverner
Time line discussion
- Need someone else on time line.
- 16th of June Lab induction
- Deadlines soon
- Change flight deadline – not 31st
Other business
- PDF mark up tools – PDF exchange viewer, foxit, keeps mark-up
- Making dropbox use better
Items for next agenda
- Need wiki transferred by next meeting
- Review of flow chart if done
Next meeting
- Thursday early as possible – email to organise
- Chair: Da, Minutes: Alan, Computer: Younus
