Team:Newcastle/3 September 2010
From 2010.igem.org
(Difference between revisions)
Shethharsh08 (Talk | contribs) (→Conclusion) |
(→Results) |
||
Line 14: | Line 14: | ||
==Results== | ==Results== | ||
- | [[Image: | + | [[Image:03.09.10.png#file|400px]] |
==Conclusion== | ==Conclusion== |
Revision as of 00:43, 26 October 2010
|
Contents |
yneA
Aims
The aim of the experiment is to test for the correct integration of yneA in pGFPrrnb plasmid.
Materials and protocols
- Please refer to: Plasmid extraction.
- Please refer to:Restriction digest. We used Nhe1 and Spe1 to remove the yneA from pGFP-rrnB.
- Please refer to: Gel electrophoresis for running all the digested plasmid fragments.
Results
Conclusion
The results show that the digest works, there is a band at approximately 541bp corresponding to yneA and a band at approximately 8.4kbp corresponding to pGFPrrnb in lanes 2,3,4,6,7,8,9, 11 and 12.