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Team:Newcastle/Meetings/26 March 2010
From 2010.igem.org
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(→4. Research Feedback) |
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| Line 9: | Line 9: | ||
====4. Research Feedback==== | ====4. Research Feedback==== | ||
| - | '''Properties of concrete and surface tension''' (Zoltan and Steven) | + | #'''Properties of concrete and surface tension''' (Zoltan and Steven) |
| - | *High compressive strength low tensile strength | + | #*High compressive strength low tensile strength |
| - | *Steal added good for temp change | + | #*Steal added good for temp change |
| - | *CaCO<sub>3</sub> – similar to concrete; expand same as concrete... might crack like concrete | + | #*CaCO<sub>3</sub> – similar to concrete; expand same as concrete... might crack like concrete |
| - | *Spider silk like steal- reduce possibility of cracking | + | #*Spider silk like steal- reduce possibility of cracking |
| - | *Glue- no many papers- expansion may not be a problem? | + | #*Glue- no many papers- expansion may not be a problem? |
| - | *Silk alkali resistant | + | #*Silk alkali resistant |
| - | *Capillary properties of concrete change over time- in project we consider small rapid | + | #*Capillary properties of concrete change over time- in project we consider small rapid |
| - | *Cement water ratio and surface area and environment | + | #*Cement water ratio and surface area and environment |
| - | *Where to look for the cracks? Crack pattern | + | #*Where to look for the cracks? Crack pattern |
| - | '''Microinjection''' (Zoltan and Steven) | + | #'''Microinjection''' (Zoltan and Steven) |
| - | *Simple issue | + | #*Simple issue |
| - | *Not correct term | + | #*Not correct term |
| - | *Swimming Bacillus | + | #*Swimming Bacillus |
| - | *Oxygen gradient big selling points | + | #*Oxygen gradient big selling points |
| - | '''Genetics Homologous recombination plasmid chromosome''' (Harsh and Rachel) | + | #'''Genetics Homologous recombination plasmid chromosome''' (Harsh and Rachel) |
| - | *Test in plasmids- Find good plasmid | + | #*Test in plasmids- Find good plasmid |
| - | *Integrate into genome | + | #*Integrate into genome |
| - | *Recombination site... Find!!! | + | #*Recombination site... Find!!! |
| - | *amyE site amylase with insert can’t break down amylase | + | #*amyE site amylase with insert can’t break down amylase |
| - | '''Nutrients''' (Harsh and Rachel) | + | #'''Nutrients''' (Harsh and Rachel) |
| - | *Bacteria algae growth living in concrete | + | #*Bacteria algae growth living in concrete |
| - | *Only activate feed bacillus | + | #*Only activate feed bacillus |
| - | *Two hybrid | + | #*Two hybrid |
| - | *Biofilms exclude other not algae | + | #*Biofilms exclude other not algae |
| - | *Antifungals, antialgaes, antibiotic | + | #*Antifungals, antialgaes, antibiotic |
| - | '''Sporulation, germination and secretion control''' (Alan and Da) | + | |
| - | *SpoA – last years team sporulation control, maybe turn off completely | + | #'''Sporulation, germination and secretion control''' (Alan and Da) |
| - | *Nutrient availability | + | #*SpoA – last years team sporulation control, maybe turn off completely |
| - | *KinA | + | #*Nutrient availability |
| - | *Inducible promoter | + | #*KinA |
| - | *Glue out of bug | + | #*Inducible promoter |
| + | #*Glue out of bug | ||
'''Quorum sensing''' (Younus and Phil) | '''Quorum sensing''' (Younus and Phil) | ||
| Line 67: | Line 68: | ||
Build it up the use. | Build it up the use. | ||
Mutations- How stop general questions | Mutations- How stop general questions | ||
| - | Not specifically a problem – keep thinking about it though | + | Not specifically a problem – keep thinking about it though |
====Action points==== | ====Action points==== | ||
Revision as of 00:43, 26 October 2010
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Contents |
Formal meeting - 26th March 2010
- Room: CBCB; Start: 11 am
- Chair: Jannetta, Minutes: Rachel, Computer: Harsh
- Present: Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh
- Apologies: Phil (Holiday), Jem and Matt
- Approval of agenda and minutes - Approved
4. Research Feedback
- Properties of concrete and surface tension (Zoltan and Steven)
- High compressive strength low tensile strength
- Steal added good for temp change
- CaCO3 – similar to concrete; expand same as concrete... might crack like concrete
- Spider silk like steal- reduce possibility of cracking
- Glue- no many papers- expansion may not be a problem?
- Silk alkali resistant
- Capillary properties of concrete change over time- in project we consider small rapid
- Cement water ratio and surface area and environment
- Where to look for the cracks? Crack pattern
- Microinjection (Zoltan and Steven)
- Simple issue
- Not correct term
- Swimming Bacillus
- Oxygen gradient big selling points
- Genetics Homologous recombination plasmid chromosome (Harsh and Rachel)
- Test in plasmids- Find good plasmid
- Integrate into genome
- Recombination site... Find!!!
- amyE site amylase with insert can’t break down amylase
- Nutrients (Harsh and Rachel)
- Bacteria algae growth living in concrete
- Only activate feed bacillus
- Two hybrid
- Biofilms exclude other not algae
- Antifungals, antialgaes, antibiotic
- Sporulation, germination and secretion control (Alan and Da)
- SpoA – last years team sporulation control, maybe turn off completely
- Nutrient availability
- KinA
- Inducible promoter
- Glue out of bug
Quorum sensing (Younus and Phil)
- CompX and cff
- Two-component system comP
Kill switch (Richard and Jannetta)
- Why ? Don’t want?- not die after job fill new cracks
- Nuclease
- Phil masEF
- Skf SbB
- If linear DNA works
Glue, CaCo3 and spider silk (Zoltan and Jannetta)
- Bacteria that produce CaCo3 from urea
- How secrete spider silk long complex proteins – how can we make it happen in bacillus
- Secretion systems
Other points
- Linear DNA – Richard
Justifications of Linear Build it up the use. Mutations- How stop general questions Not specifically a problem – keep thinking about it though
Action points
- All - Read Voygt paper
- All - Next week working off the proper wiki
- Janetta contact details doc in drop box
- Janetta and Richard – Flow chart!!!
- Reminder to neil and jen not welcome trust funding by 2nd of April?
- Janetta- T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper , quotes dependent on design
- Jannetta- send us links for graphic design software- please
- Transfer the wiki
- Individual iGEM applications - Phil hasn’t applied; Richard not accepted; Janetta text Phil
- Jen/Neil - Pay for team!!
- Visas/Passport update - Harsh will apply May/June; Richard needs to get a visa waiver
Wiki transfer
- Clarify contents of identified pages
- Ethics page
- Research page
- Safety page
- Identify other pages required
- Ideas for layout/theme
- Zoltan Tidy it up-Urgent
- Borrow last years / Look at anything thats nice
- It evolves!!! We can change it!!! Now!!!!!!!
Travel plans
- Flights and Accomodation
- Need to sort out who is going
Funding
- Harsh has made draft letter
- Main chemical company – free stuff?
Advisors/additional team members - civil engineer/graphics designer
- Still looking – Neil,Jen
Tutorials
- Sequentia and Clone manager - basics
- Modelling part two
What next?
- Flow chart of what we’re going to do Big picture!!!
- If its coloured life is easier
- Cambridge brick - Colour
- Web services for Biosynthetics, Flow chart
- Taverner
Time line discussion
- Need someone else on time line.
- 16th of June Lab induction
- Deadlines soon
- Change flight deadline – not 31st
Other business
- PDF mark up tools – PDF exchange viewer, foxit, keeps mark-up
- Making dropbox use better
Items for next agenda
- Need wiki transferred by next meeting
- Review of flow chart if done
Next meeting
- Thursday early as possible – email to organise
- Chair: Da, Minutes: Alan, Computer: Younus
