Team:Newcastle/Meetings/26 March 2010
From 2010.igem.org
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====4. Research Feedback==== | ====4. Research Feedback==== | ||
+ | |||
'''Properties of concrete and surface tension''' (Zoltan and Steven) | '''Properties of concrete and surface tension''' (Zoltan and Steven) | ||
*High compressive strength low tensile strength | *High compressive strength low tensile strength | ||
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*Cement water ratio and surface area and environment | *Cement water ratio and surface area and environment | ||
*Where to look for the cracks? Crack pattern | *Where to look for the cracks? Crack pattern | ||
+ | |||
'''Microinjection''' (Zoltan and Steven) | '''Microinjection''' (Zoltan and Steven) | ||
*Simple issue | *Simple issue | ||
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*Swimming Bacillus | *Swimming Bacillus | ||
*Oxygen gradient big selling points | *Oxygen gradient big selling points | ||
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'''Genetics Homologous recombination plasmid chromosome''' (Harsh and Rachel) | '''Genetics Homologous recombination plasmid chromosome''' (Harsh and Rachel) | ||
*Test in plasmids- Find good plasmid | *Test in plasmids- Find good plasmid | ||
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*Recombination site... Find!!! | *Recombination site... Find!!! | ||
*amyE site amylase with insert can’t break down amylase | *amyE site amylase with insert can’t break down amylase | ||
+ | |||
'''Nutrients''' (Harsh and Rachel) | '''Nutrients''' (Harsh and Rachel) | ||
*Bacteria algae growth living in concrete | *Bacteria algae growth living in concrete | ||
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*Inducible promoter | *Inducible promoter | ||
*Glue out of bug | *Glue out of bug | ||
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'''Quorum sensing''' (Younus and Phil) | '''Quorum sensing''' (Younus and Phil) | ||
*CompX and cff | *CompX and cff | ||
*Two-component system comP | *Two-component system comP | ||
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'''Kill switch''' (Richard and Jannetta) | '''Kill switch''' (Richard and Jannetta) | ||
*Why ? Don’t want?- not die after job fill new cracks | *Why ? Don’t want?- not die after job fill new cracks | ||
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*If linear DNA works | *If linear DNA works | ||
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- | Bacteria that produce CaCo3 from urea | + | '''Glue, CaCo3 and spider silk''' (Zoltan and Jannetta) |
- | How secrete spider silk long complex proteins – how can we make it happen in bacillus | + | *Bacteria that produce CaCo3 from urea |
- | Secretion systems | + | *How secrete spider silk long complex proteins – how can we make it happen in bacillus |
- | + | *Secretion systems | |
- | + | ||
- | + | '''Other points''' | |
- | + | *Linear DNA – Richard | |
Justifications of Linear | Justifications of Linear | ||
Build it up the use. | Build it up the use. |
Revision as of 00:30, 26 October 2010
|
Formal meeting - 26th March 2010
- Room: CBCB; Start: 11 am
- Chair: Jannetta, Minutes: Rachel, Computer: Harsh
- Present: Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh
- Apologies: Phil (Holiday), Jem and Matt
- Approval of agenda and minutes - Approved
4. Research Feedback
Properties of concrete and surface tension (Zoltan and Steven)
- High compressive strength low tensile strength
- Steal added good for temp change
- CaCO3 – similar to concrete; expand same as concrete... might crack like concrete
- Spider silk like steal- reduce possibility of cracking
- Glue- no many papers- expansion may not be a problem?
- Silk alkali resistant
- Capillary properties of concrete change over time- in project we consider small rapid
- Cement water ratio and surface area and environment
- Where to look for the cracks? Crack pattern
Microinjection (Zoltan and Steven)
- Simple issue
- Not correct term
- Swimming Bacillus
- Oxygen gradient big selling points
Genetics Homologous recombination plasmid chromosome (Harsh and Rachel)
- Test in plasmids- Find good plasmid
- Integrate into genome
- Recombination site... Find!!!
- amyE site amylase with insert can’t break down amylase
Nutrients (Harsh and Rachel)
- Bacteria algae growth living in concrete
- Only activate feed bacillus
- Two hybrid
- Biofilms exclude other not algae
- Antifungals, antialgaes, antibiotic
Sporulation, germination and secretion control (Alan and Da)
- SpoA – last years team sporulation control, maybe turn off completely
- Nutrient availability
- KinA
- Inducible promoter
- Glue out of bug
Quorum sensing (Younus and Phil)
- CompX and cff
- Two-component system comP
Kill switch (Richard and Jannetta)
- Why ? Don’t want?- not die after job fill new cracks
- Nuclease
- Phil masEF
- Skf SbB
- If linear DNA works
Glue, CaCo3 and spider silk (Zoltan and Jannetta)
- Bacteria that produce CaCo3 from urea
- How secrete spider silk long complex proteins – how can we make it happen in bacillus
- Secretion systems
Other points
- Linear DNA – Richard
Justifications of Linear Build it up the use. Mutations- How stop general questions Not specifically a problem – keep thinking about it though |-
|5. Action points
|- |All -Read Voygt paper |- |All-Next week working off the proper wiki!!! |- |Janetta contact details doc in drop box |- |Janetta and Richard – Flow chart!!! |- |Reminder to neil and jen not welcome trust funding by 2nd of April? |- |Janetta- T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper , quotes dependent on design |- |Janetta- send us links for graphic design software- please |- |Transfer the wiki |-
|1. Individual iGEM applications
|- |Phil hasn’t applied |- |Richard not accepted |- |Janetta text Phil |- |Jen/Neil-Pay for team!! |-
|2. Visas/Passport update
|- |Harsh- May June apply |- |Richard visa waiver |-
|3. Wiki transfer
|-
|1. Clarify contents of identified pages
|-
|2. Id Ethics page
|- |Research page |- |Safety page |- |entify other pages required |-
|3. Ideas for layout / theme
|- |Zoltan Tidy it up-Urgent |- |Borrow last years / Look at anything thats nice |- |It evolves!!! We can change it!!! Now!!!!!!! |-
|4. Travel plans
|-
|1. Flights
|- |Need to sort out who is going |-
|2. Accomodation
|- |Need to sort out who is going |-
|5. Funding
|- |Harsh has made draft letter |- |Main chemical company – free stuff ?
|-
|6. Advisors/additional team members - civil engineer/graphics designer?
|- |Still looking –Neil,Jen |-
|7. Tutorials
|- |Sequentia and Clone manager- basics |- |Modelling part two |-
|8. What next?
|- |Flow chart of what we’re going to do Big picture!!! |- |If its coloured life is easier |- |Cambridge brick-Colour |- |Web services for Biosynthetics, Flow chart |- |Deverner |-
|9. Time line discussion
|- |Need someone else on time line. |- |16th of June Lab induction |- |Deadlines soon |- |Change flight deadline – not 31st
|-
|6. Other business
|- |Pdf mark up tools – pdf exchange viewer, foxit, keeps mark up |- |Making dropbox use better |-
|7. Items for next agenda
|- |Need wiki transferred by next meeting!!! |- |Review of flow chart if done |-
|8. Next meeting- Thursday early as possible – email organise 1. Chair-Da 2. Minutes-Alan 3. Computer-Younus|-