Team:Newcastle/Meetings/26 March 2010

Formal meeting - 26th March 2010

• Room: CBCB; Start: 11 am
• Chair: Jannetta, Minutes: Rachel, Computer: Harsh
• Present: Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh
• Apologies: Phil (Holiday), Jem and Matt
• Approval of agenda and minutes - Approved

3. Brief summary of previous meeting |- |Tutorial |-

 | 4. Research Feedback

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        |1. Properties of concrete and surface tension (Zoltan and Steven)

High compressive strength low tensile strength Steal added good for temp change Caco3 – similar to concrete; expand same as concrete... might crack like concrete Spider silk like steal- reduce possibility of cracking Glue- no many papers- expansion may not be a problem? Silk alkali resistant Capillary properties of concrete change over time- in project we consider small rapid Cement water ratio and surface area and environment Where to look for the cracks? Crack pattern |-

       | 2. Microinjection (Zoltan and Steven)

|- |Simple issue Not correct term Swimming Bacillus Oxygen gradient big selling points |-

        |3. Genetics Homologous recombination plasmid chromosome (Harsh and Rachel)

|- |Test in plasmids- Find good plasmid Integrate into genome  Recombination site... Find!!! amyE site amylase with insert can’t break down amylase |-

        |4. Nutrients (Harsh and Rachel)

|- |Bacteria algae growth living in concrete Only activate feed bacillus Two hybrid Bio films exclude other not algae Antifungals, anti algals, Antibiotic |-

        |5. Sporulation, germination and secretion control (Alan and Da)

|- |SpoA – last years team sporulation control, maybe turn off completely Nutrient availability KinA Inducible promoter |- |Glue out of bug? |-

        |6. Quorum sensing (Younus and Phil)

|- |Compx And cff Two component system comp |-

        |-7. Kill switch (Richard and Jannetta)

|- |Why ? Don’t want?- not die after job fill new cracks Nuclease Phil masEF Skf SbB If linear DNA works |-

        |8. Glue, CaCo3 and spider silk (Zoltan and Jannetta)

Bacteria that produce CaCo3 from urea How secrete spider silk long complex proteins – how can we make it happen in bacillus Secretion systems |-

        |9. Other points

|- |Linear DNA – Richard Justifications of Linear Build it up the use. Mutations- How stop general questions Not specifically a problem – keep thinking about it though |-

  |5. Action points

|- |All -Read Voygt paper |- |All-Next week working off the proper wiki!!! |- |Janetta contact details doc in drop box |- |Janetta and Richard – Flow chart!!! |- |Reminder to neil and jen not welcome trust funding by 2nd of April? |- |Janetta- T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper , quotes dependent on design |- |Janetta- send us links for graphic design software- please |- |Transfer the wiki |-

   |1. Individual iGEM applications

|- |Phil hasn’t applied |- |Richard not accepted |- |Janetta text Phil |- |Jen/Neil-Pay for team!! |-

        |2. Visas/Passport update

|- |Harsh- May June apply |- |Richard visa waiver |-

        |3. Wiki transfer

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              |1. Clarify contents of identified pages

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              |2. Id Ethics page 

|- |Research page |- |Safety page |- |entify other pages required |-

              |3. Ideas for layout / theme

|- |Zoltan Tidy it up-Urgent |- |Borrow last years / Look at anything thats nice |- |It evolves!!! We can change it!!! Now!!!!!!! |-

    |4. Travel plans

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              |1. Flights

|- |Need to sort out who is going |-

              |2. Accomodation

|- |Need to sort out who is going |-

        |5. Funding

|- |Harsh has made draft letter |- |Main chemical company – free stuff ?

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        |6. Advisors/additional team members - civil engineer/graphics designer?

|- |Still looking –Neil,Jen |-

        |7. Tutorials

|- |Sequentia and Clone manager- basics |- |Modelling part two |-

        |8. What next?

|- |Flow chart of what we’re going to do Big picture!!! |- |If its coloured life is easier |- |Cambridge brick-Colour |- |Web services for Biosynthetics, Flow chart |- |Deverner |-

        |9. Time line discussion 

|- |Need someone else on time line. |- |16th of June Lab induction |- |Deadlines soon |- |Change flight deadline – not 31st

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|6. Other business

|- |Pdf mark up tools – pdf exchange viewer, foxit, keeps mark up |- |Making dropbox use better |-

  |7. Items for next agenda

|- |Need wiki transferred by next meeting!!! |- |Review of flow chart if done |-

  |8. Next meeting- Thursday early as possible – email organise 
        1. Chair-Da 
        2. Minutes-Alan
        3. Computer-Younus

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