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Team:Newcastle/3 September 2010
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==Materials and protocols== | ==Materials and protocols== | ||
| - | [[Team:Newcastle/Qiagen_Minipreps#Plasmid_extraction| Plasmid extraction]] | + | #Please refer to: [[Team:Newcastle/Qiagen_Minipreps#Plasmid_extraction| Plasmid extraction]]. |
| - | [[Team:Newcastle/Restriction_digests|Restriction digest]] | + | #Please refer to:[[Team:Newcastle/Restriction_digests|Restriction digest]]. We used EcoR1 to linearise the plasmid so that we acn run it on the gel. |
| - | [[Team:Newcastle/Gel_electrophoresis| Gel electrophoresis]] | + | #Please refer to:[[Team:Newcastle/Gel_electrophoresis| Gel electrophoresis]] for running all the digested plasmid fragments. |
==Results== | ==Results== | ||
Revision as of 23:44, 25 October 2010
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Contents |
yneA
Aims
The aim of the experiment is to test for the correct integration of yneA in pGFPrrnb plasmid.
Materials and protocols
- Please refer to: Plasmid extraction.
- Please refer to:Restriction digest. We used EcoR1 to linearise the plasmid so that we acn run it on the gel.
- Please refer to: Gel electrophoresis for running all the digested plasmid fragments.
Results
Conclusion
The results show that the digest works, there is a band at 541bp corresponding to yneA and a band at 8.4kbp corresponding to GFPrrnb in lanes 2,3,4,6,7,8,9, 11 and 12.
   
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