Team:Stockholm/13 October 2010

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I hope this will save some time since I am skipping the transformation into Top 10 cells. We don't have much time left of the competition therefore I hope this will work.
I hope this will save some time since I am skipping the transformation into Top 10 cells. We don't have much time left of the competition therefore I hope this will work.
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== Mimmi ==
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=== Home page design ===
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*Bacteria figures for each link...
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**saftey
 +
**Sponsors
 +
**Project
 +
**Planning
 +
**News
 +
**Team
 +
**Modelling
 +
**Notebook
 +
**Protocols
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*to do (?):
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**Results
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**BioBricks
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**Assemblies
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=== SOD - growth curve ===
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- ON culture
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*SOD.his
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*LMWP.SOD.his
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*TAT.SOD.his
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*Tra10.SOD.his
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 +
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*3ml Amp<sub>100</sub> + toothpick of glycerol stock

Revision as of 23:36, 25 October 2010

Home Project Idea Lab Work Results Modelling Team       Follow us on and        2010.igem.org

↳   Notebook Safety Protocols


Contents

Andreas

Plasmid prep

From 12/10 ON cultures

DNA concentration
Sample Conc [ng/μl] A260/A280
pSB1C3.IgGp 237.9 1.89
pSB1C3.bFGF 210.3 1.91
pSB1C3.ProtA 173.6 1.89
pSB1C3.yCCS 229.2 1.91
pSB1C3.SOD 193.0 1.87

Sequencing

  • 15 μl plasmid DNA; 1.5 μl primer (VR)
DNA concentration
Sample Label Sequence code
pSB1C3.IgGp pSB1C3.IgGp_VR ASB0045 776
pSB1C3.bFGF pSB1C3.bFGF_VR ASB0045 777
pSB1C3.ProtA pSB1C3.ProtA_VR ASB0045 778
pSB1C3.yCCS pSB1C3.yCCS_VR ASB0045 779
pSB1C3.SOD pSB1C3.SOD_VR ASB0045 780

Nina

concentration measurement

I measured the concentration of the gel cleaned up samples from yesterday by spectrophotometry.

Aq16.jpg

Ligation

I ligated the gel cleaned samples.

Aq17.jpg

Transformation

I transformed the ligation samples into nine BL21 tubes each with 100 ul cells. I added 6 ul of the ligations to the cells and I thawed the tubes in the first step in 15 min.

I hope this will save some time since I am skipping the transformation into Top 10 cells. We don't have much time left of the competition therefore I hope this will work.



Mimmi

Home page design

  • Bacteria figures for each link...
    • saftey
    • Sponsors
    • Project
    • Planning
    • News
    • Team
    • Modelling
    • Notebook
    • Protocols
  • to do (?):
    • Results
    • BioBricks
    • Assemblies


SOD - growth curve

- ON culture

  • SOD.his
  • LMWP.SOD.his
  • TAT.SOD.his
  • Tra10.SOD.his


  • 3ml Amp100 + toothpick of glycerol stock
Retrieved from "http://2010.igem.org/Team:Stockholm/13_October_2010"