Revision as of 21:37, 25 October 2010

Materials

**table 1**: Used kits.
Kits	Supplier	Catalog Number
HiSpeed® Plasmid Maxi Kit (25)	QIAGEN	12663
CompactPrep Plasmid Maxi Kit (25)	QIAGEN	12763
RNeasy® Mini Kit (25)	QIAGEN	74106
QIAquick® Miniprep Kit (250)	QIAGEN	27106
QIAquick® Gel Extraction Kit (250)	QIAGEN	28706
QIAquick® Nucleotid Removal Kit (250)	QIAGEN	28306
QIAquick® PCR Purification Kit (250)	QIAGEN	28106
GeneMorph® II EZC Clone Domain Mutagenesis Kit	Stratagene	200552-5

**table 2**: Used DNA ladders for gel electrophoresis.
Name	Supplier	Range
GeneRuler™ High Range DNA Ladder	Fermentas	10,171-48,502 bp
GeneRuler™ 1 kb Plus DNA Ladder	Fermentas	75-20,000 bp

NEBuffers are color-coded (NEBuffer 1-yellow, NEBuffer 2-blue, NEBuffer 3-red, NEBuffer 4-green) and supplied as 10X

**table 4**: Used *E. coli* strains.
Name	Purpose	Reference
DH5α	Amplification of Plasmids	Invitrogen
SCS110	Amplification of unmethylated Plasmids	Stratagene
Top10	Amplification of Plasmids	Invitrogen

Please find our Primers & Oligos on an extra subpage.

The following is a list of all used Instruments.

**table 6**: Used Instruments.
Description	Name	Firm	Table Centrifuge	Microfuge® 18 Centrifuge	Beckman Coulter^TM
Table Centrifuge	MicrofugeR 22® Centrifuge	Beckman Coulter^TM
Centrifuge	Allegra X-12	Beckman Coulter^TM
Shaker	VORTEXGENIER 2	Scientific Industries, SI^TM
Heating plate (magnet stirrer)	MR Hei-Standard	Heidolph
Heatblock	QBD4	Grant
Heatblock (shakeing function)	Thermomixer comfort	Eppendorf
PCR-machine	MyCycler^TM thermo cycler	BioRad
UV-chamber	Transluminator	Vilber Lourmat
Scale (fine)	Pioneer^TM PA114C	OHAUS
Scale	Pioneer^TM PA4101C	OHAUS
Fridge	KTP 1750 Premium	Liebherr
Freezer	GP 1366 Premium	Liebherr
-86 Freezer	-86°C Ultralow Freezer	NUAIRE^TM
Hood	Tischabzug	Wesemann® Laboreinrichtung
Draw-off pump	Vacuhand control	Vacubrand
Incubator	HT Multitron Version 2	INFORS
Incubator (cell culture)	HERAcell 150	Thermo electron coorperation
Hood (cell culture)	HERAsafe	Thermo electron coorperation
Plate reader	Infinite M200	TECAN
FACS	Cytomics FC 500 MPL	Beckman Coulter^TM
Microscope	CKX41	Olympus
Computer		Sun Microsystems
UV/Visivle Spectrometer	Ultrospec 3300 pro	Amersham Biosciences
Photometer	NanoDrop® ND-1000 Spectrophotometer	peQLab Biotechnologie GmbH
Photometer	NanoVue^TM	General Electric
Ice Machine	MF22	SCOTSMAN®
Gel electrophoresis chamber	Mupid®-One	Advance
pipetting robot	QIAcube^TM	QIAGEN
Wide field microscope	Nikon Eclipse 90i	Nikon
PCR machine	MyCycler thermal cycler	BIO-RAD
Real-time PCR machine	StepOnePlus^TM Real-time PCR System	Applied Biosystems^TM

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