Team:SDU-Denmark/project-i

From 2010.igem.org

(Difference between revisions)

Revision as of 21:38, 1 July 2010

The Idea

Inspired by an article on flow generated in a micro-capillary tube by a bacterial "pump" [http://microfluids.engin.brown.edu/Breuer_Papers/Journals/Small2008_Bacterial_Pump.pdf] we have decided to attempt construction of a similar system. Our approach centers on E. coli that will be modified to hyper-express flagella, in an attempt to increase force generation potential. These bacteria will be attached to a surface, and their flagella should therefor generate flow in the surrounding liquid.

Regulation of the pump will be introduced through a photo-sensing chimeric fusion protein that has recently been shown to integrate with the E. coli chemotaxis system [http://pubs.acs.org/doi/abs/10.1021/bi034399q], to control tumbling frequencies in our bacteria, thereby giving us control over the amount of turbulence disrupting our pump via a blue light source. The parts contributed will in effect confer phototaxic ability to E. coli.

We suggest that our system will also be convertible to a mixing device, for mixing fluids in micro-capillaries, and other related environments.

Both sub-project will contribute new parts to the registry.

--CKurtzhals 21:33, 1 July 2010 (UTC)

1. Microfluidic pump powered by self-organizing bacteria., Min Jun Kim and Kenneth S. Breuer; Small. 2008 January; 4(1): 111–118. doi: 10.1002/smll.200700641.

2. Photostimulation of a Sensory Rhodopsin II/HtrII/Tsr Fusion Chimera Activates CheA-Autophosphorylation and CheY-Phosphotransfer in Vitro†, Vishwa D. Trivedi and, John L. Spudich; Biochemistry 2003 42 (47), 13887-13892

Revision as of 21:34, 1 July 2010 (view source) CKurtzhals (Talk \| contribs) (→The Idea) ← Older edit		Revision as of 21:38, 1 July 2010 (view source) CKurtzhals (Talk \| contribs) (→The Idea) Newer edit →
Line 11:		Line 11:

	Regulation of the pump will be introduced through a photo-sensing chimeric fusion protein that has recently been shown to integrate with the E. coli chemotaxis system [http://pubs.acs.org/doi/abs/10.1021/bi034399q], to control tumbling frequencies in our bacteria, thereby giving us control over the amount of turbulence disrupting our pump via a blue light source. The parts contributed will in effect confer phototaxic ability to E. coli.		Regulation of the pump will be introduced through a photo-sensing chimeric fusion protein that has recently been shown to integrate with the E. coli chemotaxis system [http://pubs.acs.org/doi/abs/10.1021/bi034399q], to control tumbling frequencies in our bacteria, thereby giving us control over the amount of turbulence disrupting our pump via a blue light source. The parts contributed will in effect confer phototaxic ability to E. coli.
		+
		+
		+	We suggest that our system will also be convertible to a mixing device, for mixing fluids in micro-capillaries, and other related environments.