Team:SDU-Denmark/project-i

From 2010.igem.org

(Difference between revisions)
(References)
(The Idea)
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Inspired by an article on flow generated in a micro-capillary tube by a bacterial "pump" [[https://2010.igem.org/Team:SDU-Denmark/project-i#References 4]],
Inspired by an article on flow generated in a micro-capillary tube by a bacterial "pump" [[https://2010.igem.org/Team:SDU-Denmark/project-i#References 4]],
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we have decided to attempt construction of a similar system. In the article a pump was constructed by coating the inside of a tube with ''Serratia marcesens''. This was done by washing a bacterial suspension through the tube at a speed, that allowed the cells to adhere to the surface. The flow also had the effect of aligning most of the bacteria so their flagella were facing downstream. When the induction of flow was stopped, the bacteria kept the solution flowing with their flagella, in essence acting as a pump. Control was lent by altering the glucose concentration of the buffer solution. They were able to show a measurable force for several hours, before the cells became de-energized and died.<br><br>
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we have decided to attempt construction of a similar system. In the article a pump was constructed by coating the inside of a tube with ''Serratia marcesens''. This was done by washing a bacterial suspension through the tube at a speed, that allowed the cells to adhere to the surface. The flow also had the effect of aligning most of the bacteria so their flagella were facing downstream. When the induction of flow was stopped, the bacteria kept the solution flowing with their flagella, in essence acting as a pump. Control was lent by altering the glucose concentration of the buffer solution. They were able to show a measurable force for several hours, before the cells became de-energized and died.<br>
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[[Image:The_pump.png | 600px]]
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Revision as of 10:58, 25 October 2010