Team:Heidelberg/Project/Mouse Infection
From 2010.igem.org
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===production of recombinant virus=== | ===production of recombinant virus=== | ||
===procedure involving animals=== | ===procedure involving animals=== | ||
+ | The mouse experiments were conducted in accordance with the animal facility of the german cancer research institute of Heidelberg. Female NMRI mice were purchased from....??. At 8-10 weeks of age the animals were injected in the tail vein (TV), with ~1x10^11 particles of AAV-SV40-luciferase in 200µl of 1x phosphate-buffered saline. The mice are transferred to a holding device which restrains the mouse while allowing access to the tail vein. The tails were warmed before the injections and injections were carried out usind 27 gauge needles. All the mice recoverd from the injection quickly without loss of mobility or interruption of grooming activity. | ||
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===in vivo animal imaging=== | ===in vivo animal imaging=== | ||
Revision as of 10:47, 25 October 2010
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