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From 2010.igem.org
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| - | We present our modeling details of this AHL-induced LuxR-regulated lux pR-gfp system. There are 39 reactions in total. A screenshot is provided below: | + | We present our modeling details of this AHL-induced LuxR-regulated lux pR-gfp system. We assume: |
| + | |||
| + | *LuxR does not form dimer; | ||
| + | *AHL binds to LuxR and forms AHL-LuxR complex; | ||
| + | *AHL-LuxR could form dimer, (AHL-LuxR)2; | ||
| + | *Only (AHL-LuxR)2 could activate transcription from promoter lux pR; | ||
| + | |||
| + | There are 12 species in total: | ||
| + | *0:E_coli: E.coli cell in flask; | ||
| + | *1:AHL: AHL added in the flask; | ||
| + | *2:DNA: plasmid transformed into E.coli at the beginning; | ||
| + | *3:AHL: AHL in E.coli because of diffusion; | ||
| + | *4:RNA: mRNA of GFP; | ||
| + | *5:RNA: mRNA of LuxR; | ||
| + | *6:E0040_GFP: GFP protein; | ||
| + | *7:C0062_LuxR: LuxR protein; | ||
| + | *8:E0040_GFP_mature: mature GFP protein; | ||
| + | *9:AHL;C0062_LuxR: complex of AHL and LuxR; | ||
| + | *10:(AHL)2;(C0062_LuxR)2: dimer of complex AHL-LuxR; | ||
| + | *11:(AHL)2;(C0062_LuxR)2;DNA: binding complex of AHL-LuxR dimer and lux pR on plasmid DNA; | ||
| + | |||
| + | There are 39 reactions in total. A screenshot is provided below: | ||
[[Image:USTCs Reaction1.PNG|700px|center]] | [[Image:USTCs Reaction1.PNG|700px|center]] | ||
Revision as of 08:08, 25 October 2010
We present our modeling details of this AHL-induced LuxR-regulated lux pR-gfp system. We assume:
- LuxR does not form dimer;
- AHL binds to LuxR and forms AHL-LuxR complex;
- AHL-LuxR could form dimer, (AHL-LuxR)2;
- Only (AHL-LuxR)2 could activate transcription from promoter lux pR;
There are 12 species in total:
- 0:E_coli: E.coli cell in flask;
- 1:AHL: AHL added in the flask;
- 2:DNA: plasmid transformed into E.coli at the beginning;
- 3:AHL: AHL in E.coli because of diffusion;
- 4:RNA: mRNA of GFP;
- 5:RNA: mRNA of LuxR;
- 6:E0040_GFP: GFP protein;
- 7:C0062_LuxR: LuxR protein;
- 8:E0040_GFP_mature: mature GFP protein;
- 9:AHL;C0062_LuxR: complex of AHL and LuxR;
- 10:(AHL)2;(C0062_LuxR)2: dimer of complex AHL-LuxR;
- 11:(AHL)2;(C0062_LuxR)2;DNA: binding complex of AHL-LuxR dimer and lux pR on plasmid DNA;
There are 39 reactions in total. A screenshot is provided below:
