Team:Brown/Protocols
From 2010.igem.org
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Cell competence protocols | Cell competence protocols | ||
*[[Media:Protocol_Quick_CaCl2_competence.pdf|Quick CaCL2 Competence]] | *[[Media:Protocol_Quick_CaCl2_competence.pdf|Quick CaCL2 Competence]] | ||
+ | *[[Media:Protocol_CaCl2_competent_cells.pdf|CaCl2 competence (bulk)]] |
Revision as of 04:47, 25 October 2010
Protocols
Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our Notebook. PCR
Ligations
Restriction digests
Overnights
Transformations
Cell competence protocols