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Team:Harvard/flavor/results
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<u>Figure 1. Induced expression of YFP-tagged Miraculin and Brazzein in E. Coli</u></br> | <u>Figure 1. Induced expression of YFP-tagged Miraculin and Brazzein in E. Coli</u></br> | ||
| - | Figure 1 (A) through (D) are normalized plots of Miraculin and Brazzein YFP-fused constructs expressed in E. Coli. | + | Figure 1 (A) through (D) are normalized plots of Miraculin and Brazzein YFP-fused constructs expressed in E. Coli. <i>YFP-2x</i> tags were attached to both N- and C- termini to ensure that folding was not hindered. In all cases relative YFP fluorescence had appreciably increased after 120 minutes as compared to the non-induced <i>E. Coli</i> |
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Revision as of 02:53, 25 October 2010
Results
Coming Soon!
Confirmation with YFP-2x Tags
In order to confirm that the Miraculin and Brazzein are able to be expressed in E. Coli we attached a YFP-2x tag sequence to the termini of both proteins. The proteins were placed under an IPTG-expressible promoter and used spectrophotometry to determine the level of YFP fluorescence against a baseline, untagged protein. Figure 1 shows relative-fluorescence at times post induction. In all circumstances the levels of YFP-fluorescence increased
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Figure 1 click to enlarge 
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