Team:Harvard/flavor/notebook

From 2010.igem.org

(Difference between revisions)
(06-16-2010 [ top ])
(06-17-2010 [ top ])
Line 105: Line 105:
==<html><a class="labnotebook" name="06-17-2010">06-17-2010</a></html> [ [[#top|top]] ]==
==<html><a class="labnotebook" name="06-17-2010">06-17-2010</a></html> [ [[#top|top]] ]==
 +
===PCR Purification of pORE Vector Parts===
 +
Following QIAgen PCR Purification Kit Protocol the following PCR products were purified:
 +
#pENTCUP2 Promoter - 16.1 ng/μL
 +
#NOSterminator Sequence - 76.3 ng/μL
 +
#STOP codon + NOSterminator Sequence - 76.5 ng/μL
 +
To do: follow up with restriction digest <font color="green"> &#x2713; </font> and Agarose gel<font color="green"> &#x2713; </font> to confirm PCR and PCR purification. <!--<font color="green"> &#x2713; </font>-->
 +
 +
===Restriction Digest and Agarose Gel===
 +
Restriction Digest reactions were set up as follows:
 +
 +
pENTCUP2 Promoter:<br>
 +
*27μL PCR product<br>
 +
*3μL Loading Buffer w/ dye<br>
 +
*1μL Xba1 Fast Enzyme<br>
 +
*1μL Pst1 Fast Enzyme<br>
 +
 +
NOSterm and NOSterm + STOP:
 +
*9μL PCR product
 +
*1μL Loading Buffer w/ dye
 +
*0.5μL Xba1 Fast Enzyme
 +
*0.5μL Pst1 Fast Enzyme
 +
 +
BioBrick Plasmid V0120 (Ampicillin Resistance):
 +
*3μL DNA
 +
*1μL Loading Buffer w/ dye
 +
*6μL diH<sub>2</sub>O
 +
*0.5μL Xba1 Fast Enzyme
 +
*0.5μL Pst1 Fast Enzyme
 +
 +
Reactions were allowed to proceed for 45 minutes at 37°C.<br>
==<html><a class="labnotebook" name="06-18-2010">06-18-2010</a></html> [ [[#top|top]] ]==
==<html><a class="labnotebook" name="06-18-2010">06-18-2010</a></html> [ [[#top|top]] ]==

Revision as of 00:28, 25 October 2010



notebook calendar [ top ]

Week 1 06-14-2010 06-15-2010 06-16-2010 06-17-2010 06-18-2010
Week 2 06-21-2010 06-22-2010 06-23-2010 06-24-2010 06-25-2010
Week 3 06-28-2010 06-29-2010 06-30-2010 07-01-2010 07-02-2010
Week 4 07-05-2010 07-06-2010 07-07-2010 07-08-2010 07-09-2010
Week 5 07-12-2010 07-13-2010 07-14-2010 07-15-2010 07-16-2010
Week 6 07-19-2010 07-20-2010 07-21-2010 07-22-2010 07-23-2010
Week 7 07-26-2010 07-27-2010 07-28-2010 07-29-2010 07-30-2010
Week 8 08-02-2010 08-03-2010 08-04-2010 08-05-2010 08-06-2010
Week 9 08-09-2010 08-10-2010 08-11-2010 08-12-2010 08-13-2010

06-14-2010 [ top ]

  • Miraculin and brazzein constructs due to arrive on Wednesday from Mr. Gene

BioBrick Transformation

BioBrick parts from the 2010 iGEM kit were transformed and grown in highly competent TURBO bacteria.

  • Wintergreen Scent Pathway:

BBa_J45700 - entire pathway, Ampicillin
BBa_J45004 - BSMT1 only, Ampicillin
(Not in 2010 BB Kit: BBa_J45017 - PchB, PchA)

  • Banana Scent Pathway:

BBa_J45250 - ATF3 + Promoter, Ampicillin?
BBa_J45014 - ATF3 only, Ampicillin
(Not in BB 2010 Kit: BBa_J45400 - BAT2 and THI3)

06-15-2010 [ top ]

Primer Designs for Agrobacterium Vector

  • Primers were designed to amplify from the Expression Series pORE Agrobacterium plasmid (e3) 1)the pENTcup2 promoter, 2) the tNOS stop sequence, 3) the tNOS stop sequence + additional stop codon and 4) the pHLP promoter.

Note: the NOSterm_BB_R primer works for both tNOS and tNOS+STOP codon sequences.

   pENTcup2_BB_F
   CCTTTCTAGAGGGATCTTCTGCAAGCATCT
   pENTcup2_BB_R
   AAGGCTGCAGCGGCCGCTACTAGTTCCGGTGGGTTTTGAGGT
   STOP_NOSterm_BB_F
   CCTTTCTAGATGAGATCGTTCAAACATTTGG
   NOSterm_BB_F
   CCTTTCTAGAGATCGTTCAAACATTTGGCA
   NOSterm_BB_R
   AAGGCTGCAGCGGCCGCTACTAGTGATCTAGTAACATAGATGACA
   pHPL_BB_F
   CCTTTCTAGAAACGTGGATACTTGGCAGTG
   pHPL_BB_R
   AAGGCTGCAGCGGCCGCTACTAGTCTTTTGAGCTTAGAGGTTTTT

BioBrick Transformation

  • Colonies were observed after overnight culture growth, but in low quantities.
    • J45700 and J45004 (both Wintergreen Pathway) showed minimal number of colonies on both 10μL and 100μL cultures.
    • J45250 and J45014 (both Banana Pathway) showed no colonies on either the 10μL or 100μL cultures.

Codon Usage in Arabidopsis

Using http://gcua.schoedl.de/


Valencene Extraction

Used RNeasy Plant Mini Kit to extract RNA from the flavedo of an Organic Valencia Orange.

Flavedo

06-16-2010 [ top ]

MiniPrep

  • MiniPrep of Wintergreen parts from Registry (J45004 and J45700) following Qiagen MiniPrep protocol. MiniPrep three samples of each part.
    • DNA concentrations:
         J45004-1: 59.1 ng/μL
         J45004-2: 72.9 ng/μL
         J45004-3: 88.1 ng/μL
 
         J45700-1: 146.7 ng/μL
         J45700-2: 187.4 ng/μL
         J45700-3: 175.5 ng/μL

Digestion with Enzymes

  • For J45004, we added: 9μL DNA, 1μL buffer, 1μL xbaI restriction enzyme (slow), and .5μL pstI restriction enzyme (fast).
  • For J45700, we added 5μL DNA, 4μL H2O, 1μL buffer, 1μL xbaI restriction enzyme (slow), and .5μL pstI restriction enzyme (fast).
  • We let mixtures sit for 30 minutes due to the use of xbaI restriction enzyme (slow). After the 30 minutes, we added 2.5μL dye to each mix.

Gel

  • We loaded 12.5μL of 1kb ladder to well 1 of the gel (numbered left to right). We then loaded 12.5μL of J45004-1, J45004-2, and J45004-3 to wells 2,3, and 4, respectively. We loaded J45700-1, J45700-2, J4500-3 in wells 5, 6, and 7, respectively (see images below for well locations).
  • Ran on 1% agarose gel.

BBa_J45700 BBa_J45700

06-17-2010 [ top ]

PCR Purification of pORE Vector Parts

Following QIAgen PCR Purification Kit Protocol the following PCR products were purified:

  1. pENTCUP2 Promoter - 16.1 ng/μL
  2. NOSterminator Sequence - 76.3 ng/μL
  3. STOP codon + NOSterminator Sequence - 76.5 ng/μL

To do: follow up with restriction digest and Agarose gel to confirm PCR and PCR purification.

Restriction Digest and Agarose Gel

Restriction Digest reactions were set up as follows:

pENTCUP2 Promoter:

  • 27μL PCR product
  • 3μL Loading Buffer w/ dye
  • 1μL Xba1 Fast Enzyme
  • 1μL Pst1 Fast Enzyme

NOSterm and NOSterm + STOP:

  • 9μL PCR product
  • 1μL Loading Buffer w/ dye
  • 0.5μL Xba1 Fast Enzyme
  • 0.5μL Pst1 Fast Enzyme

BioBrick Plasmid V0120 (Ampicillin Resistance):

  • 3μL DNA
  • 1μL Loading Buffer w/ dye
  • 6μL diH2O
  • 0.5μL Xba1 Fast Enzyme
  • 0.5μL Pst1 Fast Enzyme

Reactions were allowed to proceed for 45 minutes at 37°C.

06-18-2010 [ top ]

06-21-2010 [ top ]

06-22-2010 [ top ]

06-23-2010 [ top ]

06-24-2010 [ top ]

06-25-2010 [ top ]

06-28-2010 [ top ]

06-29-2010 [ top ]

06-30-2010 [ top ]

07-01-2010 [ top ]

07-02-2010 [ top ]

07-05-2010 [ top ]

07-06-2010 [ top ]

07-07-2010 [ top ]

07-08-2010 [ top ]

07-09-2010 [ top ]

07-12-2010 [ top ]

07-13-2010 [ top ]

07-14-2010 [ top ]

07-15-2010 [ top ]

07-16-2010 [ top ]

07-19-2010 [ top ]

07-20-2010 [ top ]

07-21-2010 [ top ]

07-22-2010 [ top ]

07-23-2010 [ top ]

07-26-2010 [ top ]

07-27-2010 [ top ]

07-28-2010 [ top ]

07-29-2010 [ top ]

07-30-2010 [ top ]

08-02-2010 [ top ]

08-03-2010 [ top ]

08-04-2010 [ top ]

08-05-2010 [ top ]

08-06-2010 [ top ]

08-09-2010 [ top ]

08-10-2010 [ top ]

08-11-2010 [ top ]

08-12-2010 [ top ]

08-13-2010 [ top ]