Team:Yale/Our Project/Protocols/TSI agar

From 2010.igem.org

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<p>
<p>
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<h4> Triple Sugar Iron Agar Recipe </h4>
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<h4> Triple Sugar Iron (TSI) Agar Recipe </h4>
<ul>
<ul>
-
<p> <li>500 mL water </a></li> </p>
+
 
-
<li>1.5 g Meat Extract </a></li>
+
<li>500 mL water </li>  
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<li>1.5 g Yeast Extract</a></li>
+
<li>1.5 g Meat Extract </li>
-
<li>7.5 g Pancreatic Digest of Casein</a></li>
+
<li>1.5 g Yeast Extract</li>
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<li> 2.5 g Protease Peptone</a></li>
+
<li>7.5 g Pancreatic Digest of Casein</li>
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<li>0.5 g Dextrose</a></li>
+
<li> 2.5 g Protease Peptone</li>
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<li>0.5 g Lactose</a></li>
+
<li>0.5 g Dextrose</li>
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<li>0.5 g Sucrose</a></li>
+
<li>0.5 g Lactose</li>
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<li>0.26 g Ferrous Ammonium Sulfate (hexahydrate)</a></li>
+
<li>0.5 g Sucrose</li>
-
<li>2.5 g NaCl</a></li>
+
<li>0.26 g Ferrous Ammonium Sulfate (hexahydrate)</li>
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<li>0.15 g Sodium Thiosulfate</a></li>
+
<li>2.5 g NaCl</li>
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<li>6 g Agar</a></li>
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<li>0.15 g Sodium Thiosulfate</li>
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<li>12 mg Phenol Red</a></li>
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<li>6 g Agar</li>
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<li>+ Ampicillin</a></li>
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<li>12 mg Phenol Red</li>
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<li>+ Ampicillin</li>
 +
 
</ul>
</ul>
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Revision as of 19:34, 24 October 2010

iGEM Yale

protocols

Triple Sugar Iron (TSI) Agar Recipe

  • 500 mL water
  • 1.5 g Meat Extract
  • 1.5 g Yeast Extract
  • 7.5 g Pancreatic Digest of Casein
  • 2.5 g Protease Peptone
  • 0.5 g Dextrose
  • 0.5 g Lactose
  • 0.5 g Sucrose
  • 0.26 g Ferrous Ammonium Sulfate (hexahydrate)
  • 2.5 g NaCl
  • 0.15 g Sodium Thiosulfate
  • 6 g Agar
  • 12 mg Phenol Red
  • + Ampicillin

Retrieved from "http://2010.igem.org/Team:Yale/Our_Project/Protocols/TSI_agar"