Team:Yale/Our Project/Protocols/TSI agar

From 2010.igem.org

iGEM Yale

protocols

Triple Sugar Iron (TSI) Agar Recipe

  • 500 mL water
  • 1.5 g Meat Extract
  • 1.5 g Yeast Extract
  • 7.5 g Pancreatic Digest of Casein
  • 2.5 g Protease Peptone
  • 0.5 g Dextrose
  • 0.5 g Lactose
  • 0.5 g Sucrose
  • 0.26 g Ferrous Ammonium Sulfate (hexahydrate)
  • 2.5 g NaCl
  • 0.15 g Sodium Thiosulfate
  • 6 g Agar
  • 12 mg Phenol Red
  • + Ampicillin

Retrieved from "http://2010.igem.org/Team:Yale/Our_Project/Protocols/TSI_agar"