Team:SDU-Denmark/project-i

From 2010.igem.org

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== Retinal biosynthesis ==
== Retinal biosynthesis ==
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For the photoreceptor to work, we will need to supply it with enzymes for retinal biosynthesis. Retinal is formed by cleaving beta-carotene, a reaction that is catalyzed by beta-carotene-oxygenases [[https://2010.igem.org/Team:SDU-Denmark/project-i#References 11]]. We will be supplying a new BioBrick that expresses a beta-carotene 15'15-monooxygenase from ''Drosophila melanogaster''. Beta-carotene biosynthesis will be supplied by a part made by the [http://partsregistry.org/Part:BBa_K274210 2009 Cambrigde team ]. We will also do further characterization of the part BBa_K274210 that was made by the Cambridge tam in 2009 in new strains and with different analytical methods.
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For the photoreceptor to work, we will need to supply it with enzymes for retinal biosynthesis. Retinal is formed by cleaving beta-carotene, a reaction that is catalyzed by beta-carotene-oxygenases [[https://2010.igem.org/Team:SDU-Denmark/project-i#References 11]]. We will be supplying a new BioBrick that contains the gene ''ninaB'' from ''Drosophila melanogaster'' and expresses 15,15' beta carotene dioxygenase which cleaves beta-carotene to two molecules of retinal. Beta-carotene biosynthesis will be supplied by a part made by the [http://partsregistry.org/Part:BBa_K274210 2009 Cambrigde team ]. We will also do further characterization of the Cambridge part in new strains of ''E. coli'' and with different analytical methods.
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Revision as of 18:54, 24 October 2010