Team:Heidelberg/Notebook/Material Methods
From 2010.igem.org
(→Enzymes) |
(→Enzymes) |
||
Line 68: | Line 68: | ||
|BglI||-||50%||75%||100%||50%|| | |BglI||-||50%||75%||100%||50%|| | ||
|- | |- | ||
- | |BglII||13 10%|| 75%|| 100%|| 10%|||| | + | |BglII||13|| 10%|| 75%|| 100%|| 10%|||| |
|- | |- | ||
- | |BsrGI | + | |BsrGI||-|| 25%|| 100%|| 10%|| 100%||||x |
|- | |- | ||
- | |DpnI 6 100%|| 100%|| 75%|| 100%|| | + | |DpnI ||6|| 100%|| 100%|| 75%|| 100%|| |
|- | |- | ||
- | |DpnI | + | |DpnI ||-|| 100%|| 100%|| 75%|| 100%|| |
|- | |- | ||
- | |EcoRI | + | |EcoRI ||-|| 100%|| 100%|| 100%|| 100%|| |
|- | |- | ||
- | |EcoRV | + | |EcoRV ||-|| 50%|| 75%|| 100%|| 50%|| x |
|- | |- | ||
- | |FseI | + | |FseI ||-|| 100%|| 75%|| 0%|| 100%|| |
|- | |- | ||
- | |HindIII 10 50%|| 100%|| 10%|| 50%|| | + | |HindIII||-|| 10 50%|| 100%|| 10%|| 50%|| |
|- | |- | ||
- | |HindIII | + | |HindIII||-||50%|| 100%|| 10%|| 50%|| |
|- | |- | ||
- | |KpnI 11 100%|| 75%|| 0%|| 50%|| x | + | |KpnI ||11|| 100%|| 75%|| 0%|| 50%|| x |
|- | |- | ||
- | |MfeI-HF™ 75%|| 50%|| 10%|| 100%|| | + | |MfeI-HF™||-|| 75%|| 50%|| 10%|| 100%|| |
|- | |- | ||
- | |NarI 26 100%|| 75%|| 75%|| 100%|| | + | |NarI ||26|| 100%|| 75%|| 75%|| 100%|| |
|- | |- | ||
|NcoI ||12|| 100%|| 100%|| 100%|| 100%|| | |NcoI ||12|| 100%|| 100%|| 100%|| 100%|| | ||
Line 108: | Line 108: | ||
|SacI ||16|| 100%|| 50%|| 10%|| 100%|| | |SacI ||16|| 100%|| 50%|| 10%|| 100%|| | ||
|- | |- | ||
- | |ScaI ||18|| not not 100%|| not | + | |ScaI ||18|| not||not||100%||not|| |
|- | |- | ||
|SfcI ||19|| 75%|| 50%|| 10%|| 100%|| x | |SfcI ||19|| 75%|| 50%|| 10%|| 100%|| x | ||
Line 127: | Line 127: | ||
|- | |- | ||
|XmaI||25|| 25%|| 50%|| 0%|| 100%|| x | |XmaI||25|| 25%|| 50%|| 0%|| 100%|| x | ||
- | + | |- | |
+ | |- | ||
NEBuffers are color-coded (NEBuffer 1-yellow, NEBuffer 2-blue, | NEBuffers are color-coded (NEBuffer 1-yellow, NEBuffer 2-blue, | ||
NEBuffer 3-red, NEBuffer 4-green) and supplied as 10X | NEBuffer 3-red, NEBuffer 4-green) and supplied as 10X |
Revision as of 18:35, 24 October 2010
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