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Project/SpooA/
From 2010.igem.org
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=== BioBrick Background === | === BioBrick Background === | ||
| - | + | So as to inhibit the transcription driven by the PlcR promoter, the SpoOA protein binds to a particular sequence within the promoter. Binding to this region prevents polymerase from properly interacting with the promoter and thus represses transcription. The regions within the PlcR promoter that SpoOA binds are referred to as the SpoOA boxes. Two of these boxes flank the PlcR-PapR positive regulator binding site thus repressing transcription [1]. | |
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=== Sequence and Annotation === | === Sequence and Annotation === | ||
<br /> | <br /> | ||
=== Testing and verification === | === Testing and verification === | ||
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Revision as of 16:23, 24 October 2010
Contents |
SpooA repressor - [http://partsregistry.org/Part:BBa_K354004 BBa_K354004]
BioBrick Background
So as to inhibit the transcription driven by the PlcR promoter, the SpoOA protein binds to a particular sequence within the promoter. Binding to this region prevents polymerase from properly interacting with the promoter and thus represses transcription. The regions within the PlcR promoter that SpoOA binds are referred to as the SpoOA boxes. Two of these boxes flank the PlcR-PapR positive regulator binding site thus repressing transcription [1].
Sequence and Annotation
Testing and verification
