Team:Heidelberg/Notebook/Material Methods
From 2010.igem.org
(→Coating) |
(→Coating) |
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===Coating=== | ===Coating=== | ||
for 96-well plates | for 96-well plates | ||
- | *add 15ul of poly-L-lysine solution to each well (make sure whole surface is covered with solution) | + | *add 15ul of poly-L-lysine solution (COMPANY) to each well (make sure whole surface is covered with solution) |
*leave for 30' in the incubator | *leave for 30' in the incubator | ||
*remove poly-L-lysine solution | *remove poly-L-lysine solution | ||
- | *wash once with PBS | + | *wash once with 1x PBS |
{{:Team:Heidelberg/Pagemiddle}} | {{:Team:Heidelberg/Pagemiddle}} | ||
{{:Team:Heidelberg/Bottom}} | {{:Team:Heidelberg/Bottom}} |
Revision as of 09:44, 24 October 2010
![](https://static.igem.org/mediawiki/2010/9/92/Bg_plasmid.png)
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