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Team:Brown/Notebook/September24
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(New page: {{:Team:Brown/templates/header}} ==Friday, September 24, 2010== ===Checking on Overnights=== Came in to check on Ethan's overnights- looks like nothing grew - 8:15 AM. Perhaps due to selec...) |
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Came in to check on Ethan's overnights- looks like nothing grew - 8:15 AM. Perhaps due to selecting satellite colonies mistakenly? | Came in to check on Ethan's overnights- looks like nothing grew - 8:15 AM. Perhaps due to selecting satellite colonies mistakenly? | ||
Nothing to miniprep. | Nothing to miniprep. | ||
| + | |||
| + | ===Other Notes=== | ||
| + | ==Took plates from incubator at 9:15 AM== | ||
| + | * Tat-L in pSB1A3 plate: 5-8 colonies, will overnight later today. | ||
| + | (8:40 PM: overnight started) | ||
| + | * Tat-L in pGEM: TONS of colonies (not countable); only a small fraction are blue! Will overnight later today. | ||
| + | (8:40 PM: two overnights started) | ||
| + | |||
| + | ==Retrieved PCR samples from thermocycler, will run on gel later== | ||
| + | *Look for ~100 bp segment amplified by Tat-fwd and rev primers. If present, confirm insertion of Tat-L into pBlueScript. | ||
| + | |||
| + | ==Miniprepped overnights from 9/23== | ||
| + | *labeled "mini #1 JH 9/24" | ||
| + | |||
| + | ===Overnight Cultures=== | ||
| + | 15 overnights of big colonies of AraC, CI, Mnt as before. | ||
| + | *Colony PCR on corresponding colonies | ||
| + | 50 μl total: | ||
| + | *25 μl mix | ||
| + | *1.5 each primer (2x1.5) -- later realized this was 10x too much primer | ||
| + | *22 μl dH2O | ||
| + | |||
| + | PCR started at 1:38 PM. | ||
===5:35 PM=== | ===5:35 PM=== | ||
Put 15 culture tubes in the incubator. | Put 15 culture tubes in the incubator. | ||
Revision as of 04:41, 24 October 2010
Friday, September 24, 2010
Checking on Overnights
Came in to check on Ethan's overnights- looks like nothing grew - 8:15 AM. Perhaps due to selecting satellite colonies mistakenly? Nothing to miniprep.
Other Notes
Took plates from incubator at 9:15 AM
- Tat-L in pSB1A3 plate: 5-8 colonies, will overnight later today.
(8:40 PM: overnight started)
- Tat-L in pGEM: TONS of colonies (not countable); only a small fraction are blue! Will overnight later today.
(8:40 PM: two overnights started)
Retrieved PCR samples from thermocycler, will run on gel later
- Look for ~100 bp segment amplified by Tat-fwd and rev primers. If present, confirm insertion of Tat-L into pBlueScript.
Miniprepped overnights from 9/23
- labeled "mini #1 JH 9/24"
Overnight Cultures
15 overnights of big colonies of AraC, CI, Mnt as before.
- Colony PCR on corresponding colonies
50 μl total:
- 25 μl mix
- 1.5 each primer (2x1.5) -- later realized this was 10x too much primer
- 22 μl dH2O
PCR started at 1:38 PM.
5:35 PM
Put 15 culture tubes in the incubator.
