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Team:Brown/Notebook/September20
From 2010.igem.org
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J13002-LacI Ligation: remainder of DNA added for second attempt transformation of XL1B. | J13002-LacI Ligation: remainder of DNA added for second attempt transformation of XL1B. | ||
| - | + | ===Overnight Culture=== | |
Started overnight culture for Arac-J13002 ligation. | Started overnight culture for Arac-J13002 ligation. | ||
(3 μl Amp in 3 mL LB) | (3 μl Amp in 3 mL LB) | ||
Latest revision as of 23:11, 23 October 2010
Monday, September 20, 2010
Results of transformations
- AraC-J13002 ligation --> 4 to 5 colonies
- LacI-J13002 ligation --> 0 transformants
Will start overnight culture later today for AraC-J13002 ligation transformants.
J13002-LacI Ligation: remainder of DNA added for second attempt transformation of XL1B.
Overnight Culture
Started overnight culture for Arac-J13002 ligation. (3 μl Amp in 3 mL LB) picked 3 colonies.
11:05 PM - placed in 37°C.
