Team:Lethbridge/Project/Compartamentalization
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- | <p><font color="white">( | + | <p><font color="white">(C)</p> |
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- | <th> | + | <th><p><font color="white">(D) coming soon!</p> |
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- | <font color="white">Figure 2</p> | + | <font color="white">Figure 2. Theoretical lumazine synthase (LS) and fluorescent protein (FP) expression under different IPTG and arabinose (ara) concentrations. (A) Increasing concentrations of both ara and IPTG. As the concentration of IPTG increases the production of LS increases; as the concentration of ara increases the production of FPs decreases. (B) When initially induced with IPTG and ara, the production of LS would increase over time. The production of FPs would not occur until the ara is degraded by the cell (reducing it’s concentration), then the production of FPs would increase. (C) When there is no ara present the production of FP would immediately occur. LS production would not occur until IPTG was present. The conditions shown in B and C would allow us to determine the optimal expression conditions to get the maximum localization of protein within the compartment. We will determine this by using FRET between CFP and YFP. |
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