Team:Northwestern/Project/Lac

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Modeling	Chassis	Induction	Chitin	Apoptosis

Induction

Induction System

For the induction system, our team decided to use the lac-operon/iptg construct because of its widespread use and the availability of information. This system was assembled using standard biobricks parts. To induce our system, we used a IPTG spray of two and five millimolar concentrations, and one millimolar solutions for liquid cultures.

Parts and Assembly

The induction construct consisted of a constitutive promoter (J23100, J23104, or J23105), a combination part of Ribosome Binding Site, Lac Repressor, double Terminators, and Lac Promoter (Q01121, Q04121), and Ribosome Binding Sequence (B0034, B0031, B0032). See the parts section for more information.

Multiple versions of each part were used to generate a wide range of repressor concentrations, so as to allow for variation between the induction time-delay and degree of Apoptosis protein and Chitin Synthase synthesis.

The effectiveness of these constructs were tested in the modeling section.

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 ===Induction System===
 For the induction system, our team decided to use the lac-operon/iptg construct because of its widespread use and the availability of information. This system was assembled using standard biobricks parts. To induce our system, we used a IPTG spray of two and five millimolar concentrations, and one millimolar solutions for liquid cultures.