Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test20agosto

From 2010.igem.org

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(→RESULTS (?))
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12-4C5)
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12-4C5)
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==RESULTS (?)==
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==RESULTS==
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Data were analyzed following the [[Team:UNIPV-Pavia/Parts/Characterization#Data analysis for self-inducible promoters (initiation-treshold determination)| data analysis procedure]] to evaluate the ODstart. The plots correpsoning to the parts tested are reported:
 +
 
 +
[[Image:UNIPV_Pavia_FluoVSODHC2008.png| 620px | center ]]
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 +
[[Image:UNIPV_Pavia_FluoVSODCo2008.png| 620px | center ]]
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[[Image:UNIPV_Pavia_FluoVSODLC2008.png| 620px | center ]]
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The ODstart are reported in the table below:
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{| border='1' align='center'
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|| Part || ODstart
 +
|-
 +
|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> || 0.1655
 +
|-
 +
|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> || -
 +
|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> || -
 +
|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> || 0.5421
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|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> || 0.4837
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> || 0.1503
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> || 0.3587
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> || -
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> || 0.1729
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> || -
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> || -
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> || -
 +
|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> || -
 +
|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> || -
 +
|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> || -
 +
|}
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Revision as of 16:36, 23 October 2010
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AUGUST, 20TH


PLATE


UNIPV Pavia piastra20agosto.png


EXPERIMENT DESCRIPTION

Motivation

The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.

Methods

The experiment was performed in LB according to Protocol 1 on the following measurement parts:

  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12)
  • <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name I15-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12-4C5)

RESULTS

Data were analyzed following the data analysis procedure to evaluate the ODstart. The plots correpsoning to the parts tested are reported:

UNIPV Pavia FluoVSODHC2008.png
UNIPV Pavia FluoVSODCo2008.png
UNIPV Pavia FluoVSODLC2008.png

The ODstart are reported in the table below:

Part ODstart
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> 0.1655
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> -
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> -
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> 0.5421
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> 0.4837
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> 0.1503
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> 0.3587
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> 0.1729
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> -



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