Team:Newcastle/solution

From 2010.igem.org

(Difference between revisions)
(→Required BioBricks)
Line 3: Line 3:
=== Required BioBricks ===
=== Required BioBricks ===
-
BioBrick 1 ([[Team:Newcastle/Urease|CaCO<sub>3</sub>/Urease]])
+
[[Team:Newcastle/Urease|CaCO<sub>3</sub>/Urease]]
-
BioBrick 2 ([[Team:Newcastle/End of crack & signalling system|End of crack & signalling system]])
+
[[Team:Newcastle/Filamentous Cells|Filamentous Cells]]
-
BioBrick 3 ([[Team:Newcastle/Non-target-environment kill switch|Non-target-environment kill switch]])
+
[[Team:Newcastle/End of crack & signalling system|End of crack & signalling system]]
-
BioBrick 4 ([[Team:Newcastle/Spider silk|Spider silk]] & [[Team:Newcastle/Filamentous Cells|Filamentous Cells]])
+
[[Team:Newcastle/Thick cell walls|Thick cell walls]]
 +
 
 +
[[Team:Newcastle/Non-target-environment kill switch|Non-target-environment kill switch]]
 +
 
 +
[[Team:Newcastle/Spider silk|Spider silk]]
=== Characteristics / Actions ===
=== Characteristics / Actions ===

Revision as of 16:32, 25 June 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Contents

Required BioBricks

CaCO3/Urease

Filamentous Cells

End of crack & signalling system

Thick cell walls

Non-target-environment kill switch

Spider silk

Characteristics / Actions

  • Alkaline resistant
  • Motility
  • Sense Oxygen (for cracks)
  • Quorum Sensing
  • UV Sensitivity
  • Produce glue/Silk
  • Produce product in response to signal

Products

  • Spider silk
  • Glue
  • Amyloid Fiber (forms Beta-Sheet)
  • Calcium Carbonate
  • Quorum Sensing Peptides
  • Surfactin

Priorities

  • Filamentous cells
  • CaCO3
  • Quorum Sensing
  • Spider silk
  • Glue
  • Directed movement

Types of Bacillus cells

  • Motile rod cells
  • Filamentous
  • Chain of rods
  • Spores
  • Lysed
  • Biofilm
  • Dividing cell

Environment

  • Fermenter (Batch)
  • External Face of Concrete
  • Inside Crack
  • Spray
  • End of crack
  • Non-target

Experiments

  1. Movement into cracks
  2. Speed of swimming in a particular direction

Models

  1. Rate of migration down micro crack
  2. Rate of protein production
  3. Mechanical model of repair strength
  4. Whole process. Signal propagation to release glue


Parameters

  1. Speed of swimming in a particular direction (wt)
  2. Quantities of glue, s.s, CaCO3, cell density
  3. Strength (tensile) of bonding agents, filamentous cells

 ???

  1. Grown in fermenter, rich medium
    • Rods
  2. Sporulation medium
    • spores
  3. Spray canister
    • spores
  4. Sprayed on concrete surface
    • germinate -> rods
  5. In crack
    • rods
  6. End of crack
    • Produce glue/CaCO3/silk/filaments
    • Produce signal peptides
Retrieved from "http://2010.igem.org/Team:Newcastle/solution"