Team:St Andrews/project/laboratory/protocols

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(Difference between revisions)

Revision as of 15:11, 23 October 2010 (view source) JWTaylor (Talk | contribs) (→Annealing of Complementary Primers) ← Older edit		Revision as of 15:12, 23 October 2010 (view source) JWTaylor (Talk | contribs) (→Construction of BioBricks) Newer edit →
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-	Gingko Bioworks - [http://ginkgobioworks.com/support/ Three Antibiotic Assembly (NEB Biobrick Assembly Kit)]	+	Gingko Bioworks - [http://ginkgobioworks.com/support/ 3A Biobrick Assembly] (NEB Biobrick Assembly Kit)
-	OpenWetWare [http://openwetware.org/wiki/In-fusion_biobrick_assembly In Fusion Biobrick Assembly (Primer design and assembly strategy)]	+	OpenWetWare [http://openwetware.org/wiki/In-fusion_biobrick_assembly In Fusion Biobrick Assembly] (Primer design and assembly strategy)
-	Clontech [http://www.clontech.com/products/detail.asp?catalog_id=639621 In Fusion Cloning (In Fusion reaction itself)]	+	Clontech [http://www.clontech.com/products/detail.asp?catalog_id=639621 In Fusion Cloning] (In Fusion reaction itself)
	OpenWetWare [http://openwetware.org/wiki/Endy:Annealing_complementary_primers Endy - Annealing Complementary Primers]		OpenWetWare [http://openwetware.org/wiki/Endy:Annealing_complementary_primers Endy - Annealing Complementary Primers]

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Revision as of 15:12, 23 October 2010

University of St Andrews iGEM 2010

Welcome!

University of St Andrews iGEM 2010

Our first year at iGEM!

Protocols

We used a number of protocols from OpenWetWare in addition to the manufacturer's recommended protocols for our materials.

Cell Culture

OpenWetWare [http://openwetware.org/wiki/Bacterial_cell_culture Bacterial Cell Culture]

Transformation

NEB Turbo Competent E. coli

Clontech Stellar Competent E. coli.

Restriction Digest

Promega Enzymes

NEB Biobrick Assembly Kit (Gingko Bioworks)

Ligation

NEB Biobrick Assembly Kit (Gingko Bioworks)

PCR

OpenWetWare [http://openwetware.org/wiki/Knight:Colony_PCR Knight - Colony PCR]

NEB Taq

Finnzymes Phusion

DNA Extraction

Quiagen Miniprep Isopropanol Extraction of small DNA fragments

Construction of BioBricks

Gingko Bioworks - [http://ginkgobioworks.com/support/ 3A Biobrick Assembly] (NEB Biobrick Assembly Kit)

OpenWetWare [http://openwetware.org/wiki/In-fusion_biobrick_assembly In Fusion Biobrick Assembly] (Primer design and assembly strategy)

Clontech [http://www.clontech.com/products/detail.asp?catalog_id=639621 In Fusion Cloning] (In Fusion reaction itself)

OpenWetWare [http://openwetware.org/wiki/Endy:Annealing_complementary_primers Endy - Annealing Complementary Primers]