Team:St Andrews/project/laboratory/protocols

From 2010.igem.org

(Difference between revisions)
(New page: {{:Team:St_Andrews/defaulttemplate}})
Line 1: Line 1:
{{:Team:St_Andrews/defaulttemplate}}
{{:Team:St_Andrews/defaulttemplate}}
 +
 +
==Protocols==
 +
 +
We used a number of protocols from OpenWetWare in addition to the manufacturer's recommended protocols for our materials.
 +
 +
===Cell Culture===
 +
OpenWetWare [http://openwetware.org/wiki/Bacterial_cell_culture Bacterial Cell Culture]
 +
===Transformation===
 +
 +
NEB Turbo Competent E. coli
 +
 +
Clontech Stellar Competent E. coli.
 +
 +
===Restriction Digest===
 +
 +
Promega Enzymes
 +
 +
NEB Biobrick Assembly Kit (Gingko Bioworks)
 +
 +
===Ligation===
 +
 +
NEB Biobrick Assembly Kit (Gingko Bioworks)
 +
 +
===PCR===
 +
 +
OpenWetWare [http://openwetware.org/wiki/Knight:Colony_PCR Knight - Colony PCR]
 +
 +
NEB Taq
 +
 +
Finnzymes Phusion
 +
 +
===DNA Extraction===
 +
Quiagen Miniprep
 +
Isopropanol Extraction of small DNA fragments
 +
 +
===Annealing of Complementary Primers===
 +
 +
OpenWetWare [http://openwetware.org/wiki/Endy:Annealing_complementary_primers Endy - Annealing Complementary Primers]

Revision as of 14:59, 23 October 2010


St Andrews from East Sands

University of St Andrews iGEM 2010

Welcome!

The Saints

University of St Andrews iGEM 2010

Our first year at iGEM!


Contents

Protocols

We used a number of protocols from OpenWetWare in addition to the manufacturer's recommended protocols for our materials.

Cell Culture

OpenWetWare [http://openwetware.org/wiki/Bacterial_cell_culture Bacterial Cell Culture]

Transformation

NEB Turbo Competent E. coli

Clontech Stellar Competent E. coli.

Restriction Digest

Promega Enzymes

NEB Biobrick Assembly Kit (Gingko Bioworks)

Ligation

NEB Biobrick Assembly Kit (Gingko Bioworks)

PCR

OpenWetWare [http://openwetware.org/wiki/Knight:Colony_PCR Knight - Colony PCR]

NEB Taq

Finnzymes Phusion

DNA Extraction

Quiagen Miniprep Isopropanol Extraction of small DNA fragments

Annealing of Complementary Primers

OpenWetWare [http://openwetware.org/wiki/Endy:Annealing_complementary_primers Endy - Annealing Complementary Primers]

Retrieved from "http://2010.igem.org/Team:St_Andrews/project/laboratory/protocols"