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Team:Purdue/Notebook
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===6/23/10=== | ===6/23/10=== | ||
Agar plates poured. Seeds to be sewn on 6/24 to give time for the media to solidify. | Agar plates poured. Seeds to be sewn on 6/24 to give time for the media to solidify. | ||
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| + | ===6/24/10=== | ||
| + | Seeds sown onto agar. Five large culture plates and two small plates were then transfered into horticulture growth chamber. Seeds in soil media forecasted to germinate by 6/26. | ||
Revision as of 15:37, 25 June 2010
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Contents |
Notebook
6/16/10
Prepared nine pots of wildtype (Col-0) A. thaliana and moved to cold storage for stratification. Pots intended for use in Agrobacterium flower-dip. Seeds were sewn in soilless media and the pot was covered with a wide meshing to retain the soil.
6/21/10
Transfered pots from cold storage to greenhouse. Prepared 0.5 MS agar media and sterilized seeds for sterile growth, to be sewn on 6/23. Seeds left in dilute agar solution in fridge to stratify.
6/23/10
Agar plates poured. Seeds to be sewn on 6/24 to give time for the media to solidify.
6/24/10
Seeds sown onto agar. Five large culture plates and two small plates were then transfered into horticulture growth chamber. Seeds in soil media forecasted to germinate by 6/26.
