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Team:BCCS-Bristol/Wetlab/Part Design/BioBricks/PyeaR+LacZ
From 2010.igem.org
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| - | The original plan was to use the [http://partsregistry.org/Part:BBa_K216009 BBa_K216009] part to characterise the PyeaR promoter, through a β-galactosidase assay to determine the activity of the | + | The original plan was to use the [http://partsregistry.org/Part:BBa_K216009 BBa_K216009] part to characterise the PyeaR promoter, through a β-galactosidase assay to determine the activity of the PyeaR part. Having struggled to get a workable result through Edinburgh's biobrick, we decided to set about designing our own. |
| + | |||
| + | The plan would be to take the [http://partsregistry.org/Part:BBa_K216005 BBa_K216005] part and use this as a base for the new part containing a full functional copy of the LacZ part - for this part an ideal biobrick in the registry already existed, containing a good Ribosomal Binding Site (RBS) plus the full length LacZ sequence: [http://partsregistry.org/Part:BBa_I732017 BBa_I732017]. Combining the two existing biobricks into one should have been a simple procedure, though through error and plain bad luck, after to consecutive attempts no working product was forthcoming, and with just two weeks before the new term was due to start we abandoned the construction of the PyeaR + LacZ construct in favour of getting the best data we could from Edinburgh's [http://partsregistry.org/Part:BBa_K216009 BBa_K216009] part. | ||
Revision as of 13:29, 23 October 2010
iGEM 2010
PyeaR + LacZ Construct
The original plan was to use the [http://partsregistry.org/Part:BBa_K216009 BBa_K216009] part to characterise the PyeaR promoter, through a β-galactosidase assay to determine the activity of the PyeaR part. Having struggled to get a workable result through Edinburgh's biobrick, we decided to set about designing our own.
The plan would be to take the [http://partsregistry.org/Part:BBa_K216005 BBa_K216005] part and use this as a base for the new part containing a full functional copy of the LacZ part - for this part an ideal biobrick in the registry already existed, containing a good Ribosomal Binding Site (RBS) plus the full length LacZ sequence: [http://partsregistry.org/Part:BBa_I732017 BBa_I732017]. Combining the two existing biobricks into one should have been a simple procedure, though through error and plain bad luck, after to consecutive attempts no working product was forthcoming, and with just two weeks before the new term was due to start we abandoned the construction of the PyeaR + LacZ construct in favour of getting the best data we could from Edinburgh's [http://partsregistry.org/Part:BBa_K216009 BBa_K216009] part.
