Team:SDU-Denmark/protocols

From 2010.igem.org

(Difference between revisions)
(Scanning Electron Microscope)
(CP1.1)
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• 0.25 µL TAQ polymerase enzyme '''(add just before PCR run)'''<br><br>
• 0.25 µL TAQ polymerase enzyme '''(add just before PCR run)'''<br><br>
Total vol.: 25 µL <br><br>
Total vol.: 25 µL <br><br>
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The TAQ polymerase has no proofreading, and should therefore only be used for size determination of DNA fragments. When PCR-product is to be purified and used for further experiments always use Phu polymerase!!!
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The TAQ polymerase has no proofreading, and should therefore only be used for size determination of DNA fragments. When PCR-product is to be purified and used for further experiments always use Pfu polymerase!!!
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Make enough premix for your number of colonies +3. <br><br>
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Make enough premix for your number of colony +1. <br><br>
''Protocol''
''Protocol''
<br>
<br>
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1. Select and transfer a single colony to a PCR tube with a pipette tip (afterwards use the same for plating out on plates)<br><br>
1. Select and transfer a single colony to a PCR tube with a pipette tip (afterwards use the same for plating out on plates)<br><br>
2. Add all of the H2O used in the premix to the PCR tubes and place them in the microwave at full power for 2 min. with an open lid. <br><br>
2. Add all of the H2O used in the premix to the PCR tubes and place them in the microwave at full power for 2 min. with an open lid. <br><br>
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3. Make the premix (without water). Do not add enzyme until just before premix is added to the PCR tubes. Mix the premix by pipetting up and down (do not vortex!)<br><br>
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3. Make the premix (without water). Do not add enzyme until just before premix is added to the PCR tubes. Mix the premix by pipetting up and down (do not vortex!) <br><br>
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4. Add premix to each PCR tube. <br><br>
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4. Add premix to each PCR tube.  
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<br><br>
5. Run PCR <br><br>
5. Run PCR <br><br>
PCR of DNA in solutions: <br><br>
PCR of DNA in solutions: <br><br>
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</p>
</p>
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=== CP1.2 ===
=== CP1.2 ===
<p style="text-align: justify;">
<p style="text-align: justify;">

Revision as of 13:09, 23 October 2010