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Revision as of 12:53, 23 October 2010
Primers
On this page we list all the primers we designed and used during the summer. All primers were kindly provided by [http://www.dna-technology.dk/ DNA Technology].
In the following, coding sequences are colored blue, restriction sites are colored green and nucleotides that are both are shown in orange.
The mutation sites in the FlhDC mutation primers are shown in purple.
| Primer
name |
Sequence |
Tm
(C) |
| FlhDC
fw |
5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTGAAA-3' |
88.3 |
| FlhDC
rv |
5'-GTTTCTTCCTGCAGCGGCCGCTACTATTAAACAGCCTGTACTCTCTGTTC-3' |
88.3 |
| FlhDC
mutation fw* |
5'-TTGGCAGCTTTGCCCGCAGCTTATG-3' |
61.0 |
| FlhDC
mutation rv* |
5'-CATAAGCTGCGGGCAAAGCTGCCAA-3' |
61.0 |
| Photosensor
fw |
5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGTGGGACTTACGACCTC-3' |
73.1 |
| Photosensor
rv |
5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATCAGAAGGTTTCCCAGTTCGCATC-3' |
73.9 |
| NinaB
fw |
5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGCAGCCGGTGTCTTCAAGAG-3' |
73.1 |
| NinaB
rv |
5'-GTGTCTTCCTGCAGCGGCCGCTACTAGTACTAAATGGCATTGGGTGCAAACCATC-3' |
72.4 |
| NinaB
2 fw** |
5'-CCGCTTCTAGATGGCAGCCGGTGTCTTCAAGAG-3' |
68.1 |
| NinaB
2 rv** |
5'-CGCTACTAGTACTAAATGGCATTGGGTGCAAACCATC-3' |
65.6 |
(*) Mutation primers were designed to introduce a silent mutation at position 822 in the operon to eliminate an illegal PstI site.
(**) Due to the formation of hairpin structures of primers ninaB fw and ninaB rv, new primers were designed containing only the innermost restriction sites (XbaI and SpeI).
Everything you need to get the party started!
"
