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Team:Warsaw/Stage1/ExpressIt
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<li>just one step to clone any protein coding BioBrick and you have expression construct ready</li> | <li>just one step to clone any protein coding BioBrick and you have expression construct ready</li> | ||
<li>wide spectrum of expression strengths</li> | <li>wide spectrum of expression strengths</li> | ||
| - | <li>ampicillin resistance (new variants of E. coli BL21 are chloramphenicol resistant)</li> | + | <li>ampicillin resistance (new variants of <i>E.coli</i> BL21 are chloramphenicol resistant)</li> |
<li>high copy ColE1 ori (based on <a href="http://partsregistry.org/Part:pSB1A2">pSB1A2</a> vector)</li> | <li>high copy ColE1 ori (based on <a href="http://partsregistry.org/Part:pSB1A2">pSB1A2</a> vector)</li> | ||
<li>terminator included in vector sequence</li> | <li>terminator included in vector sequence</li> | ||
Revision as of 12:34, 23 October 2010
ExpressIt! - family of expression vectors with defined strength
Key features:
- 10 different measured RBS variants
- each variant in both constitutive (J23100 driven) and inducible (pT7 driven) version
- just one step to clone any protein coding BioBrick and you have expression construct ready
- wide spectrum of expression strengths
- ampicillin resistance (new variants of E.coli BL21 are chloramphenicol resistant)
- high copy ColE1 ori (based on pSB1A2 vector)
- terminator included in vector sequence
Relative strength of ExpressIt! vector family:
