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Team:TU Delft/21 June 2010 content
From 2010.igem.org
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|2,0 μL | |2,0 μL | ||
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| - | |EcoRI | + | |EcoRI (1 U/μg) |
|1,0 μL | |1,0 μL | ||
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| - | |PstI | + | |PstI (1 U/μg) |
|1,0 μL | |1,0 μL | ||
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Revision as of 13:42, 24 June 2010
We had some drawbacks today. The gel extraction kit didn’t work as well as we had hoped, it turned out to be the DNA disappearing kit. Tomorrow a new day.
Lab work
Restriction digestion of plasmid backbone pSB1T3:
| pSB1T3 (~1,0 μg) | 2,5 μL |
| Buffer H (Roche) | 2,0 μL |
| EcoRI (1 U/μg) | 1,0 μL |
| PstI (1 U/μg) | 1,0 μL |
| H2O | 13,5 μL |
Cut digested pSB1T3 from gel and isolate the band.
