Team:TU Delft/18 June 2010 content

From 2010.igem.org

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Pieter and Eva worked on the emulsifying activity assay. Four samples were tested:
Pieter and Eva worked on the emulsifying activity assay. Four samples were tested:
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Sample #, Amount of SDS, Hexalkane
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{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
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|Sample  
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1. Blank, 0
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|Amount of SDS
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|Hexalkane
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2. No SDS, 0.02 ml
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|-
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|1
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3. 0,01% SDS, 0.02 ml
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|Blank
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|0
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4. 1% SDS, 0.02 ml
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|-
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|2
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|0% SDS
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|0.02 mL
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|-
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|3
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|0.01% SDS
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|0.02 mL
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|-
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|4
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|1% SDS
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|0.02 mL
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|}
Tris buffer (pH 8) was added to a total volume of 7.5 ml.
Tris buffer (pH 8) was added to a total volume of 7.5 ml.
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Absorbance measured at 600 nm.
Absorbance measured at 600 nm.
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1. 0
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{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
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|Sample
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2. 0
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|A600
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|-
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3. 0.4
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|1
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|0
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4. 0.2
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|-
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|2
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|0
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|-
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|3
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|0.4
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|-
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|4
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|0.2
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|}

Revision as of 13:37, 24 June 2010

We had a nice slow day, finishing some small experiments and reorganizing our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy.

We also booked a hotel in Boston!

Lab work

Pieter and Eva worked on the emulsifying activity assay. Four samples were tested:

Sample Amount of SDS Hexalkane
1 Blank 0
2 0% SDS 0.02 mL
3 0.01% SDS 0.02 mL
4 1% SDS 0.02 mL

Tris buffer (pH 8) was added to a total volume of 7.5 ml.

Incubate 1h at 30C at shaken at 200 RPM.

Absorbance measured at 600 nm.

Sample A600
1 0
2 0
3 0.4
4 0.2
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