"
Team:TU Delft/21 June 2010 content
From 2010.igem.org
(Difference between revisions)
(New page: We had some drawbacks today. The gel extraction kit didn’t work as well as we had hoped. Tomorrow a new day.) |
|||
| Line 1: | Line 1: | ||
| + | We had some drawbacks today. The gel extraction kit didn’t work as well as we had hoped, it turned out to be the DNA disappearing kit. Tomorrow a new day. | ||
| - | + | ||
| + | '''Lab work''' | ||
| + | |||
| + | Restriction digestion of plasmid backbone pSB1T3: | ||
| + | |||
| + | {| style="color:black; background-color:white;" cellpadding="10" cellspacing="0" border="1" | ||
| + | |pSB1T3 (~1,0 μg) | ||
| + | |2,5 μL | ||
| + | |- | ||
| + | |Buffer H (Roche) | ||
| + | |2,0 μL | ||
| + | |- | ||
| + | |EcoRI | ||
| + | |1,0 μL | ||
| + | |- | ||
| + | |PstI | ||
| + | |1,0 μL | ||
| + | |- | ||
| + | |H2O | ||
| + | |13,5 μL | ||
| + | |} | ||
Revision as of 13:05, 24 June 2010
We had some drawbacks today. The gel extraction kit didn’t work as well as we had hoped, it turned out to be the DNA disappearing kit. Tomorrow a new day.
Lab work
Restriction digestion of plasmid backbone pSB1T3:
| pSB1T3 (~1,0 μg) | 2,5 μL |
| Buffer H (Roche) | 2,0 μL |
| EcoRI | 1,0 μL |
| PstI | 1,0 μL |
| H2O | 13,5 μL |
