Team:UNIPV-Pavia/Freezer

From 2010.igem.org

(Difference between revisions)
Line 170: Line 170:
pSB1AK3
pSB1AK3
</td><td>
</td><td>
-
Seq WAITING(BMR); length ok
+
Seq ok(BMR); length ok
</td>  
</td>  
<td></td><td></td>
<td></td><td></td>
Line 187: Line 187:
J63009 (Amp)
J63009 (Amp)
</td><td>
</td><td>
-
length ok
+
not sequenced, length ok
</td>  
</td>  
<td></td><td></td>
<td></td><td></td>
Line 204: Line 204:
J63009 (Amp)
J63009 (Amp)
</td><td>
</td><td>
-
Seq WAITING(BMR); length ok
+
Seq ok(BMR), with an additional barcode (25bp) after the stop codon of mOrange; length ok
</td>  
</td>  
<td></td><td></td>
<td></td><td></td>
Line 223: Line 223:
pSB1AK3
pSB1AK3
</td><td>
</td><td>
-
Seq WAITING(BMR); Cm resistance ok; length ok.
+
Seq ok(BMR); Cm resistance ok; length ok.
</td>  
</td>  
<td></td><td></td>
<td></td><td></td>
Line 241: Line 241:
pSB1AK3
pSB1AK3
</td><td>
</td><td>
-
Cm resistance ok
+
not sequenced; Cm resistance ok
</td>  
</td>  
<td></td><td></td>
<td></td><td></td>
Line 259: Line 259:
pSB1AK3
pSB1AK3
</td><td>
</td><td>
-
Seq WAITING(BMR); length ok
+
Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok
</td>  
</td>  
<td></td><td></td>
<td></td><td></td>
Line 293: Line 293:
pSB1AK3
pSB1AK3
</td><td>
</td><td>
-
none
+
not sequenced; length ok
</td>  
</td>  
<td></td><td></td>
<td></td><td></td>
Line 310: Line 310:
pSB1AK3
pSB1AK3
</td><td>
</td><td>
-
Seq WAITING(BMR); length ok
+
Seq ok(BMR); length ok
</td>  
</td>  
<td></td><td></td>
<td></td><td></td>
Line 327: Line 327:
pSB1A2
pSB1A2
</td><td>
</td><td>
-
Seq WAITING(BMR); Cm resistance ok; length ok
+
Seq ok(BMR); Cm resistance ok; length ok
</td>  
</td>  
<td></td><td></td>
<td></td><td></td>
Line 344: Line 344:
pSB1A3
pSB1A3
</td><td>
</td><td>
-
Seq WAITING(BMR); length ok
+
Seq LONG PART(BMR), but the ligation worked and the sequenced portions are ok; length ok
</td>  
</td>  
<td></td><td></td>
<td></td><td></td>
Line 361: Line 361:
pSB1A3
pSB1A3
</td><td>
</td><td>
-
none
+
not sequenced
</td>  
</td>  
<td></td><td></td>
<td></td><td></td>

Revision as of 12:50, 24 June 2010
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Freezer Management



Biobrick or
ligation name		DescriptionCreation dateStrainVectorQuality controlLengthStore

<partinfo>BBa_E2050</partinfo>

mOrange, 744bp

08/06/2010

E. coli DH5-alpha

pSB2K3

Seq ok(MIT); orange pellet when contained in pSB2K3

<partinfo>BBa_K165018</partinfo>

NLS-ADH1 terminator, 253 bp (Silver Standard 5' and 3')

08/06/2010

E. coli DH5-alpha

J63009 (Amp)

Seq partially confirmed(MIT) because it lacks a T at the 3', but the ADH1 terminator (i.e. <partinfo>BBa_K165010</partinfo>) is ok. The correct ApE file is here.

<partinfo>BBa_K165037</partinfo>

tef2 promoter, 403bp

08/06/2010

E. coli DH5-alpha

pSB1AK3

Seq ok(MIT)

<partinfo>BBa_P1004</partinfo>

Chloramphenicol resistence
cassette, 769 bp

08/06/2010

E. coli DH5-alpha

pSB1A1

Seq ok(MIT 2009); length ok

<partinfo>BBa_J61001</partinfo>

R6K origin, 406bp

08/06/2010

E. coli DH5-alpha

pSB1A2

Seq ok(MIT)

<partinfo>BBa_K081008</partinfo>

RBS-luxI, 664bp

08/06/2010

E. coli DH5-alpha

pSB1A2

Seq ok(MIT); length ok

<partinfo>BBa_K125500</partinfo>

GFP fusion brick, 718bp (Silver Standard 5', Knight Standard 3')

08/06/2010

E. coli DH5-alpha

pSB1A2

Seq partially confirmed(MIT) for unknown reasons: the alignment is fully correct. The ApE file with the correct prefix and suffix is here. Length ok.

I0-1

<partinfo>BBa_K125500</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

pSB1AK3

Wrong clone

I0-2

<partinfo>BBa_K125500</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

pSB1AK3

Seq ok(BMR); length ok

I1-1 (not used)

<partinfo>BBa_E2050</partinfo> (E-S)
+
<partinfo>BBa_K165018</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

J63009 (Amp)

not sequenced, length ok

I1-2

<partinfo>BBa_E2050</partinfo> (E-S)
+
<partinfo>BBa_K165018</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

J63009 (Amp)

Seq ok(BMR), with an additional barcode (25bp) after the stop codon of mOrange; length ok

I2-1

<partinfo>BBa_P1004</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

pSB1AK3

Seq ok(BMR); Cm resistance ok; length ok.

I2-2 (not used)

<partinfo>BBa_P1004</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

pSB1AK3

not sequenced; Cm resistance ok

I3-1

<partinfo>BBa_K081008</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

pSB1AK3

Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok

I3-2

<partinfo>BBa_K081008</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

pSB1AK3

Wrong clone

I4-1 (not used)

<partinfo>BBa_K165037</partinfo> (S-P)
+
I1 (X-P)

18/06/2010

E. coli DH5-alpha

pSB1AK3

not sequenced; length ok

I4-2

<partinfo>BBa_K165037</partinfo> (S-P)
+
I1 (X-P)

18/06/2010

E. coli DH5-alpha

pSB1AK3

Seq ok(BMR); length ok

I5

I2 (E-S)
+
<partinfo>BBa_J61001</partinfo> (E-X)

18/06/2010

E. coli DH5-alpha

pSB1A2

Seq ok(BMR); Cm resistance ok; length ok

I6-2

I3 (E-S)
+
<partinfo>BBa_T9002</partinfo> (E-X)

18/06/2010

E. coli DH5-alpha

pSB1A3

Seq LONG PART(BMR), but the ligation worked and the sequenced portions are ok; length ok

I6-3 (not used)

I3 (E-S)
+
<partinfo>BBa_T9002</partinfo> (E-X)

18/06/2010

E. coli DH5-alpha

pSB1A3

not sequenced

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