Problem

Bacilla Filla repairing cracks in concrete

Concrete Background

Deena's concrete lecture provides some basic knowledge on concrete as well as questions from our presentation at iGEM UK Get Together.

Reasons cracks are bad

1. Allow water to reach and corrode steel reinforcements
2. Weaken concrete structures
3. Difficult to repair

Work in this area has already been started: [http://www.youtube.com/watch?v=PyBR3PDPa-c Bio-concrete] and a non-biological method of [http://ace-mrl.engin.umich.edu/| self healing concrete]

Those projects mentioned above are different from ours because they only allow bacteria to repair cracks that form almost instantly after concrete sets, which are cracks formed in new buildings, whereas our Bacilla Filla can repair cracks that were formed in existing structures. This makes a lot of difference to the construction industry because it can halt the process of corrosion in steel reinforcements in existing structures. Also, our cells would die after a certain period of time after repairing the crack thus making Bacilla Filla environmentally friendly.

How our project is helpful

1. Reduces corrosion rate of the steel reinforcements
2. Reduces the need to demolish and re-build pre-existing concrete structures
3. Reduces the need for cement production
4. Reduces carbon dioxide emission
5. Reduces consumption of energy resources

How our project is novel

1. Our project focuses on existing concrete structures
2. All the cells will produce calcium carbonate which has similar properties to concrete
3. Filamentous Bacillus subtilis cells have similar properties to the synthetic fibres used in fibre-reinforced concrete, and thereby act as reinforcements within the crack.
4. Getting the BacillaFilla to produce glue which will hold everything together and will fill up the crack
5. Environmental kill switch
6. We are working with a well known strain: Bacillus subtilis 168 (previous work required specific stains)

5th August 2010

Concrete Splitting Test

On the 5th of August, the whole team went down to the Structures Lab of Cassie Building that houses the School of Civil Engineering and Geosciences, to do tensile splitting test on a concrete cylinder. We tried to understand the problems of crack formation on the concrete structures and how our project BacillaFilla will help.

More photos

Dr. Colin Davie showing properties of concrete	How tension form cracks within the concrete	Concrete in compression testing machine
Teaching properties of concrete	Compression testing machine	Dr. Colin Davie carrying cracked concrete	Concrete in compression testing machine
Lab assistant showing how concrete cracked

Materials used to mix concrete

Coarse aggregates

Fine aggregates

Cement