Team:Caltech/Week 2

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Revision as of 22:58, 22 June 2010

iGEM 2010

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Transformed additional bricks from distribution with other antibiotic resistances onto plates & liquid culture.
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_I15008 I15008], [http://partsregistry.org/wiki/index.php?title=Part:BBa_I15009 I15009], [http://partsregistry.org/wiki/index.php?title=Part:BBa_I15010 I15010], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0033 E0033], [http://partsregistry.org/wiki/index.php?title=Part:BBa_M30109 M30109], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112000 K112000], [http://partsregistry.org/wiki/index.php?title=Part:BBa_J09250 J09250]

Transformation success is ambiguous - the plates are covered in a lawn of bacteria, apparently indicating an issue with the antibiotic selection. This implies either a problem with the stock solution of antibiotic (Kan/Strep), or the procedure of plating the antibiotics on the surface of the LB-agar plates.
- To find out what happened, we set up a simple experiment:
Reperformed the transformations on two of the bricks & plated on LB-agar plates with the antibiotic incorporated.

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@@ Line 8: / Line 8: @@
 ==Tuesday 6/22==
+* Transformation success is ambiguous - the plates are covered in a lawn of bacteria, apparently indicating an issue with the antibiotic selection. This implies either a problem with the stock solution of antibiotic (Kan/Strep), or the procedure of plating the antibiotics on the surface of the LB-agar plates.
+** To find out what happened, we set up a simple experiment:
+* Reperformed the transformations on two of the bricks & plated on LB-agar plates with the antibiotic incorporated.
 ==Wednesday 6/23==