Team:Imperial College London/Software Tool

From 2010.igem.org

(Difference between revisions)
Line 16: Line 16:
   var det = new Array();
   var det = new Array();
   det[1] = "This was our primary target. Read our wiki to find out more!";
   det[1] = "This was our primary target. Read our wiki to find out more!";
-
   det[2] = "test2";
+
   det[2] = "Detecting HIV Protease with our system could be a quick, simple and cheap alternative way to diagnose HIV.";
-
   det[3] = "test3";
+
   det[3] = "Currently, a lumbar puncture is necessary to diagnose the devastating Chagas' disease. However, a simple blood test could test for a specific protease called cruzipain which is produced by the Trypanasoma cruzi parasite.";
-
   det[4] = "test4";
+
   det[4] = "Detecting this coagulation factor could be a way of determining if patients should be given warfarin or a low molecular weight heparin (LMWH) if they are displaying atrial fibrilation following a stroke. Factor X is the first member of the thrombin pathway which essentially leads to blood clotting.";
   det[5] = "test5";
   det[5] = "test5";
   var prefix = "blank";
   var prefix = "blank";
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   var cleavage = new Array();
   var cleavage = new Array();
   cleavage[1] = "AGCTGGCCTCTT";
   cleavage[1] = "AGCTGGCCTCTT";
-
   cleavage[2] = "blank";
+
   cleavage[2] = "TCTCAAAACTACCCTATCGTTCAA";
-
   cleavage[3] = "blank";
+
   cleavage[3] = "AAAAAAGTTAAAGCTAAAAAA";
-
   cleavage[4] = "blank";
+
   cleavage[4] = "ATCGACGGACGT";
   cleavage[5] = "blank";
   cleavage[5] = "blank";
   var aip = "GAA ATGCGCCTTAGCAAATTCTTCAGGGACTTCATTCTTCAAAGGAAAAAA";
   var aip = "GAA ATGCGCCTTAGCAAATTCTTCAGGGACTTCATTCTTCAAAGGAAAAAA";
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|style="width:860px;background:#d5d5d5;border: solid 20px #d5d5d5;" colspan="3"|'''<html>
|style="width:860px;background:#d5d5d5;border: solid 20px #d5d5d5;" colspan="3"|'''<html>
-
<div id="sequenceout"><span id="stem">Awaiting sequence generation... </span><span id="spre" style="color:#F6CF39">test</span><span id="spro" style="color:#FC8E42"></span><span id="srbs" style="color:#D34649"></span><span id="scwb" style="color:#AB6197"></span><span id="slin" style="color:#6273A8"></span><span id="scle" style="color:#6A9ECD"></span><span id="saip" style="color:#68904E"></span><span id="ster" style="color:#9DB742"></span><span id="ssuf" style="color:#F6CF39"></span></div>
+
<div id="sequenceout"><span id="stem">Awaiting sequence generation... </span><span id="spre" style="color:#F6CF39"></span><span id="spro" style="color:#FC8E42"></span><span id="srbs" style="color:#D34649"></span><span id="scwb" style="color:#AB6197"></span><span id="slin" style="color:#6273A8"></span><span id="scle" style="color:#6A9ECD"></span><span id="saip" style="color:#68904E"></span><span id="ster" style="color:#9DB742"></span><span id="ssuf" style="color:#F6CF39"></span></div>
</html>'''
</html>'''
|-
|-

Revision as of 10:35, 20 October 2010

Software Tool
We realised early on that our detection module could be designed with a sensitivity to different proteases. By changing the cleavage site the system can accept a wide variety of inputs. This tool is designed to facilitate a quick custom sequence generation of the entire surface protein construct.
Select Protease Description

This was our primary target. Read our wiki to find out more!

Awaiting sequence generation...

Yellow - Biobrick Prefix/Suffix

Orange - Promoter

Red - Ribosome Binding Site

Purple - Cell Wall Binding Domain

Dark Blue - Adjustable Linker

Light Blue - Protease Cleavage Site

Dark Green - Autoinducing Peptide

Light Green - Terminator