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Team:TU Delft/Project/rbs-characterization/parts
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==BioBricks, the making of== | ==BioBricks, the making of== | ||
BBa_I13401 was PCR amplified using the universal primers G00100 and G00101. The purified PCR product was assembled into one of the Anderson RBS plasmids provided in the distribution plates by means of 2-way ligations. | BBa_I13401 was PCR amplified using the universal primers G00100 and G00101. The purified PCR product was assembled into one of the Anderson RBS plasmids provided in the distribution plates by means of 2-way ligations. | ||
Revision as of 09:04, 20 October 2010
BioBricks, the making of
BBa_I13401 was PCR amplified using the universal primers G00100 and G00101. The purified PCR product was assembled into one of the Anderson RBS plasmids provided in the distribution plates by means of 2-way ligations.
Transformation into competent Top10 E.coli cells yielded positives, as determined by fluorescence analysis.
The cells were cultured over 18 hours in 96-well plates using a Gen5 fluorescence and absorbance plate reader, LB with ampicillin was the culture medium used in these experiments. Only the results obtained during exponential growth phase were taken into account.
Parts
Our constructs used for measurements: <partinfo>K398500 Icon</partinfo>
<partinfo>K398501 Icon</partinfo>
<partinfo>K398502 Icon</partinfo>
<partinfo>K398503 Icon</partinfo>
<partinfo>K398504 Icon</partinfo>
