Team:TU Delft/20 September 2010 content

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(Alkane degradation)
(Alkane degradation)
 
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==Meeting minutes==
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*Hugo - Maybe some issues with the measurements over the weekend – Thursday on Friday measurement went really well, those results can be uploaded. This was done with different concentrations of hexane
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*Nadine – Salt tolerance, measured over the weekend (with LB and M9, concentrations of 0.6-0.9M NaCl)
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*Hugo is going to do the characterization of alkane degradation; Friday, Ramon will help Hugo in the lab with GC measurements
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*Do a digestion check of 017?
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*Eva: 1C3 issues – grow and isolate again
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*Luke: Pathway maps have been made coupled to MFA for on the wiki, but he should also explain the graphs in words (not only graphs)
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**It should be good to have a page that summarizes all the results, on which you can click to more information/details
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*Jelmer: Software tool – almost done, results will be linked to prefoldin
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*Nadine: Survey on the 25th, no plan yet for where, but questions already thought up, but Dirk Stemerding will
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==Alkane degradation==
==Alkane degradation==

Latest revision as of 20:43, 19 October 2010

Meeting minutes

  • Hugo - Maybe some issues with the measurements over the weekend – Thursday on Friday measurement went really well, those results can be uploaded. This was done with different concentrations of hexane
  • Nadine – Salt tolerance, measured over the weekend (with LB and M9, concentrations of 0.6-0.9M NaCl)
  • Hugo is going to do the characterization of alkane degradation; Friday, Ramon will help Hugo in the lab with GC measurements
  • Do a digestion check of 017?
  • Eva: 1C3 issues – grow and isolate again
  • Luke: Pathway maps have been made coupled to MFA for on the wiki, but he should also explain the graphs in words (not only graphs)
    • It should be good to have a page that summarizes all the results, on which you can click to more information/details
  • Jelmer: Software tool – almost done, results will be linked to prefoldin
  • Nadine: Survey on the 25th, no plan yet for where, but questions already thought up, but Dirk Stemerding will

Alkane degradation

For some reason, 009 and 010 just don't want to get into one plasmid. As a last resort we'll try every possible way to get the two together.

Digestions:

Lane # BioBrick Cut with Extra Buffer BSA Gel ok?
0 Smartladder (5μl)
D 1 009A EcoRI, SpeI AseI Buffer 2 (NEB) 1x BSA OK
D 2 009A SpeI, PstI Phosphatase Buffer 2 (NEB) 1x BSA OK
D 3 009A SpeI, PstI none Buffer 2 (NEB) 1x BSA OK
D 4 009A EcoRI, XbaI none Buffer 2 (NEB) 1x BSA OK
D 5 009A XbaI, PstI AseI Buffer 2 (NEB) 1x BSA OK
D 6 010A EcoRI, SpeI AseI Buffer 2 (NEB) 1x BSA OK
D 7 010A SpeI, PstI none Buffer 2 (NEB) 1x BSA OK
8 010A EcoRI, XbaI Phosphatase Buffer 2 (NEB) 1x BSA OK
D 9 010A EcoRI, XbaI none Buffer 2 (NEB) 1x BSA OK
D 10 010A XbaI, PstI AseI Buffer 2 (NEB) 1x BSA OK

Ligations:

'# 'Piece 1 Piece 2
028 L1 D1 D8
028 L2 D1 D9
028 L3 D2 D10
028 L4 D3 D10
028 L5 D4 D6
028 L6 D5 D7