Team:Calgary/4 June 2010

From 2010.igem.org

(Difference between revisions)
(New page: ‘’’Friday June 4, 2010’’’ Today, we observed the plates that we transformed overnight into Top10 competent cells. Again, we had trouble growing the I0500 (14N, Plate 1) from t...)
Line 1: Line 1:
-
‘’’Friday June 4, 2010’’’
+
<html xmlns="http://www.w3.org/1999/xhtml" lang="en" xml:lang="en">
 +
 
 +
<!--Colors Blue:4AC6F7, Yellow:F3F778, Green:9ECF3E, White:FFFFFF, Dark Brown:30251A-->
 +
 
 +
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" />
 +
</head>
 +
 
 +
 +
<style type="text/css">
 +
 
 +
body{
 +
margin:0;
 +
padding:0;
 +
line-height: 1.5em;
 +
}
 +
 
 +
 
 +
#maincontainer{
 +
width: 960px; /*Width of main container*/
 +
margin: 0 auto; /*Center container on page*/
 +
}
 +
 
 +
#topsection{
 +
background: #30251A;
 +
height: 90px; /*Height of top section*/
 +
}
 +
 
 +
#topsection h1{
 +
margin: 0;
 +
padding-top: 15px;
 +
                        color:white;
 +
}
 +
 
 +
#contentwrapper{
 +
float: left;
 +
width: 100%;
 +
}
 +
 
 +
#contentcolumn{
 +
padding-top: 15px;
 +
border-right: #FDE95E 170px solid;
 +
}
 +
 
 +
#sidebar{
 +
float: left;
 +
width: 170px; /*Width of right column*/
 +
margin-left: -170px; /*Set left margin to -(RightColumnWidth) */
 +
background: #FDE95E;
 +
padding-top: 15px;
 +
}
 +
 
 +
#footer{
 +
clear: left;
 +
width: 100%;
 +
background: #30251A;
 +
color: #FFF;
 +
text-align: center;
 +
padding: 4px 0;
 +
}
 +
 
 +
#footer a{
 +
color: #FFFF80;
 +
}
 +
 
 +
.innertube{
 +
margin: 10px; /*Margins for inner DIV inside each column (to provide padding)*/
 +
margin-top: 0;
 +
}
 +
 
 +
#sidebar h3{
 +
font-size: 14px;
 +
}
 +
 
 +
</style>
 +
 
 +
<body>
 +
<div id="maincontainer">
 +
<div id="topsection">
 +
<div class="innertube">
 +
<h1>University of Calgary</h1>
 +
</div>
 +
</div>
 +
 
 +
<div id="contentwrapper">
 +
<div id="contentcolumn">
 +
<div class="innertube">
 +
</html>
 +
'''Friday June 4, 2010'''
Today, we observed the plates that we transformed overnight into Top10 competent cells. Again, we had trouble growing the I0500 (14N, Plate 1) from the original well. However, with the new well (20B, Plate 3), there were two colonies of growth. Then, we did presentations on different systems containing possible promoters that could be used to initiate transcription including the Cpx system (Alex), the Spy system (Dev), the ipbAB system (Emily), sigma E system (Chris), DegP system (Jeremy), GroE system (Himika), and the team wiki (Patrick). After, we designed a possible plasmid for testing translation and transcription with our team advisors (Dave and Paul) as well as the lab technician (Deirdre). We divided up the areas and assigned each person a different area to conduct work for the summer temporarily.
Today, we observed the plates that we transformed overnight into Top10 competent cells. Again, we had trouble growing the I0500 (14N, Plate 1) from the original well. However, with the new well (20B, Plate 3), there were two colonies of growth. Then, we did presentations on different systems containing possible promoters that could be used to initiate transcription including the Cpx system (Alex), the Spy system (Dev), the ipbAB system (Emily), sigma E system (Chris), DegP system (Jeremy), GroE system (Himika), and the team wiki (Patrick). After, we designed a possible plasmid for testing translation and transcription with our team advisors (Dave and Paul) as well as the lab technician (Deirdre). We divided up the areas and assigned each person a different area to conduct work for the summer temporarily.
 +
<html>
 +
</div>
 +
</div>
 +
</div>
 +
 +
<div id="sidebar">
 +
<div class="innertube">
 +
 +
<h3>Check Another Date:</h3>
 +
 +
<table>
 +
<tr>
 +
<td>
 +
</html>
 +
{| style="background:#F3F778;"
 +
|{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2010 | month=05 | title=Team:Calgary}}
 +
|}
 +
<html>
 +
</td>
 +
 +
<tr>
 +
 +
<td>
 +
</html>
 +
{| style="background:#F3F778;"
 +
|{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2010 | month=06 | title=Team:Calgary}}
 +
|}
 +
<html>
 +
</td>
 +
 +
<tr>
 +
 +
<td>
 +
</html>
 +
{| style="background:#F3F778;"
 +
|{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2010 | month=07 | title=Team:Calgary}}
 +
|}
 +
<html>
 +
</td>
 +
 +
<tr>
 +
 +
<td>
 +
</html>
 +
{| style="background:#F3F778;"
 +
|{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2010 | month=08 | title=Team:Calgary}}
 +
|}
 +
<html>
 +
</td>
 +
 +
</tr>
 +
 +
<td>
 +
</html>
 +
{| style="background:#F3F778;"
 +
|{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2010 | month=09 | title=Team:Calgary}}
 +
|}
 +
<html>
 +
</td>
 +
 +
<tr>
 +
 +
<td>
 +
</html>
 +
{| style="background:#F3F778;"
 +
|{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2010 | month=10 | title=Team:Calgary}}
 +
|}
 +
<html>
 +
<tr>
 +
</table>
 +
</div>
 +
</div>
 +
 +
<div id="footer">
 +
<a href="http://www.ucalgary.ca">&copy;2010 University of Calgary</a>
 +
</div>
 +
 +
</div>
 +
</body>
 +
</html>

Revision as of 22:54, 21 June 2010

University of Calgary

Friday June 4, 2010

Today, we observed the plates that we transformed overnight into Top10 competent cells. Again, we had trouble growing the I0500 (14N, Plate 1) from the original well. However, with the new well (20B, Plate 3), there were two colonies of growth. Then, we did presentations on different systems containing possible promoters that could be used to initiate transcription including the Cpx system (Alex), the Spy system (Dev), the ipbAB system (Emily), sigma E system (Chris), DegP system (Jeremy), GroE system (Himika), and the team wiki (Patrick). After, we designed a possible plasmid for testing translation and transcription with our team advisors (Dave and Paul) as well as the lab technician (Deirdre). We divided up the areas and assigned each person a different area to conduct work for the summer temporarily.

Retrieved from "http://2010.igem.org/Team:Calgary/4_June_2010"