Team:Imperial College London/Modelling/Output/Objectives

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Department of Bioengineering

Division of Molecular Biosciences

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Objectives; Detailed Description; Parameters & Constants; Results & Conclusion;Download MatLab Files;

Output Amplification Model

Objectives

Our novel concept of amplifying our output by incorporating enzymes raised some important questions that needed to be answered:

How beneficial is it to incorporate the enzyme amplification step? We need to compare the speed of response between transcription and translation with 1- or 2-step ampification.
How many amplification steps are beneficial? Will the addition of further amplification steps introduce considerable time delays?
Which enzymes should be used? TEV or HIV1?

In order to answer these questions, we would have to model this system. The results of the computer models would enable us to decide which design would be the most efficient one. This design would then be put forward to be built in labs.

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   <a href="https://2010.igem.org/wiki/index.php?title=Team:Imperial_College_London/Modelling/Output/Objectives"><b>Objectives</b></a>; <a href="https://2010.igem.org/Team:Imperial_College_London/Modelling/Output/Detailed_Description"><b>Detailed Description</b></a>; <a href="https://2010.igem.org/Team:Imperial_College_London/Modelling/Output/Parameters_and_Constants"><b>Parameters & Constants</b></a>; <a href="https://2010.igem.org/Team:Imperial_College_London/Modelling/Output/Results_and_Conclusion"><b>Results & Conclusion</b></a>;<a href="https://2010.igem.org/Team:Imperial_College_London/Modelling/Output/Download_MatLab_Files"><b>Download MatLab Files</b></a>;
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+|style="font-family: helvetica, arial, sans-serif;font-size:2em;color:#ea8828;"|Output Amplification Model
+|-
+|<html><h2>Objectives</h2></html>Our novel concept of amplifying our output by incorporating enzymes raised some important questions that needed to be answered:
+#How beneficial is it to incorporate the enzyme amplification step? We need to compare the speed of response between transcription and translation with 1- or 2-step ampification.
+#How many amplification steps are beneficial? Will the addition of further amplification steps introduce considerable time delays?
+#Which enzymes should be used? TEV or HIV1?
+In order to answer these questions, we would have to model this system. The results of the computer models would enable us to decide which design would be the most efficient one. This design would then be put forward to be built in labs.
 |}